ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

The I-J Puzzle (1987)

Murphy, D B

Palo Alto, Calif. : Annual Reviews

Annual Review of Immunology 5 (1987), S. 405-427

add to mindlist on the mindlist

Details

ISSN: 0732-0582

Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff

Topics: Biology , Medicine

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1146/annurev.iy.05.040187.002201

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Tubulin subunit sorting: Analysis of the mechanisms involved in the segregation of unique tubulin isotypes within microtubule copolymers (1988)

Murphy, D. B.

Springer

Protoplasma 145 (1988), S. 176-181

add to mindlist on the mindlist

Details

ISSN: 1615-6102

Keywords: Tubulin ; Microtubules ; Tubulin isotypes ; Tubulin subunit sorting

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Vertebrate cells contain biochemical and genetic isotypes of tubulin which are expressed in unique combinations in different tissues and cell types. To determine if mixtures of tubulin isotypes assemblein vitro to form different classes of microtubules, we analyzed the composition of microtubule copolymers assembled from mixtures of chicken brain and erythrocyte tubulin. During microtubule elongation brain tubulin assembled onto the ends of microtubules faster than erythrocyte tubulin, resulting in copolymers with continually changing ratios of isotypes along their lengths. Unlike examples of microtubule assembly where the rate of polymerization depends on the association rate constant (k+) and the subunit concentration, the rate and extent of sorting in copolymers appear to depend on the dissociation rate constant (k−), which governs the rate at which subunits are released from tubulin oligomers and microtubules and thereby made available for reassembly into copolymers. The type of microtubule seed used to initiate elongation was also found to influence the composition of copolymers, indicating that polymerization favors association of subunits of the same isotype.