ISSN:
1432-1831
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abtract Five different methods for the identification of significant yeast from clinical specimens were compared for their reliability, rapidity and cost-effectiveness. Three commercial methods consisted of semi-automated Abbott's yeasts identification system using MS-2 (Abbott Laboratories, Diagnostic Division, Irving, Texas), API 20C (Analytab Products, Inc., Plainview, NY) and Uni-Yeast-Tek (Flow Labs, Inc., MacLean, VA). Two conventional methods included the modified dye-plur plate auxanographic and rapid tube assimilation method. The 242 coded clinical isolates used in this study included 20 species ofCandida, Cryptococcus, Saccharomyces Geotrichum, Rhodotorula, Torulopsis andTrichosporon. The identificaiton accuracies with all the systems ranges between 92.3% to 97.5%. Results were available with Abbott's MS-2 within 24 h, with rapid tube assimilation method in 6–48 h and in 72 h with other systems. Rapid tube assimilation and dye-pour-plate auxanographic methods were least expensive, with labour and material costing around $ 1.00 per identification, whereas the commercial system cost a little over $ 5.00.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02126052
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