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Digitale Medien

Indole alkaloid production by hairy root cultures of Catharanthus roseus (1989)

Toivonen, L. ; Balsevich, J. ; Kurz, W. G. W.

Springer

Plant cell, tissue and organ culture 18 (1989), S. 79-93

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ISSN: 1573-5044

Schlagwort(e): Agrobacterium rhizogenes ; Catharanthus roseus ; hairy root cultures ; indole alkaloids

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Hairy root cultures of Catharanthus roseus were established by infection with six different Agrobacterium rhizogenes strains. Two plant varieties were used and found to exhibit significantly different responses to infection. Forty-seven hairy root clones derived from normal plants and two derived from the flowerless variety were screened for their growth and indole alkaloid production. The growth rate and morphological appearance showed wide variations between the clones. The alkaloid spectra observed were qualitatively but not quantitatively very similar to that of the corresponding normal plant roots. No vindoline or deacetyltransferase activity could be detected in any of the cultures studied. O-acetylval-lesamine, an alkaloid which has not been previously observed in C. roseus was identified from extracts of hairy root clone No. 8. Two root clones were examined for their growth and alkaloid accumulation during a 26-day culture period. Alkaloid accumulation parallelled growth in both clones with ca. 2 mg ajmalicine and catharanthine per g dry weight being observed.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00033467

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Digitale Medien

Studies on growth and cardenolide production of Digitalis lanata tissue cultures (1989)

Tuominen, U. ; Toivonen, L. ; Kauppinen, V. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 33 (1989), S. 558-562

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Details

ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides. The possibility of using a statistical (Box-Wilson) experimental design to study the effects of four different variables on growth, differentiation, and cardenolide production of D. lanata tissue cultures are investigated. The results of the analyses were processed by linear regression analysis. Mathematical models explaining the effects of the variables were developed. The concentration of maltose and the NO3-—NH4+ ratio were found to be significant variables for both growth and cardenolide production. The size of the inoculum was also important.

Zusätzliches Material: 4 Ill.