Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Possible role of cell surface insulin degrading enzyme in cultured human lymphocytes (1987)
    Springer
    Diabetologia 30 (1987), S. 27-32 
    Details
    ISSN: 1432-0428
    Keywords: Insulin degrading enzyme ; insulin receptor ; internalization ; cultured human lymphocytes ; down-regulation ; immunoenzymatic labeling ; cell surface protease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The kinetic changes of insulin receptors and cell surface insulin degrading enzyme were examined in Bri-7 cultured human lymphocytes after preincubation with or without insulin. The concentration of cell surface insulin degrading enzyme was determined by immunoenzymatic labeling method using a polyclonal antiserum to insulin degrading enzyme. In Bri-7 cells preincubated with 10−10 to 10−5mol/l insulin for 18h, the surface insulin receptors and insulin degrading enzyme decreased progressively as a function of the concentration of insulin in the preincubation medium. The surface insulin receptors and insulin degrading enzyme of cells preincubated with 10−6mol/l insulin were decreased to 25 and 35% of the control respectively. In Bri-7 cells preincubated with 10−6 mol/l insulin for 30 min to 18 h, the loss of surface insulin degrading enzyme was slightly slower than that of the receptors; however, the curves were essentially parallel to each other. Thus, the treatment of Bri-7 cells with insulin caused down-regulation of insulin receptors in a dose- and time-dependent manner. Cell surface insulin degrading enzyme also decreased simultaneously. A combination of several insulin degradation assays (trichloroacetic acid precipitation, gel filtration and receptor rebinding) demonstrated that cell surface bound insulin remained intact, and that the degradation in Bri-7 cells seemed to be a limiting proteolysis of insulin. Furthermore, by the receptor rebinding method insulin degrading activity in cells after preincubation with 10−6 mol/l insulin (19.6±4.6%) was decreased, although not significantly, as compared with cells after preincubation without insulin (24.6±4.8%). These results suggest a possible hypothesis that cell surface insulin degrading enzyme may be internalized with the insulin-receptor complex, and that it may degrade insulin during the intracellular process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01788903
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Conditions favouring doubled haploid breeding over conventional breeding of self-fertilizing crops (1987)
    Springer
    Euphytica 36 (1987), S. 441-453 
    Details
    ISSN: 1573-5060
    Keywords: Self-fertilizing crops ; haploid ; doubled haploid ; breeding method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Monte Carlo computer simulation was used to investigate the conditions favouring doubled haploid breeding over conventional breeding of self-fertilizing crops. Two different systems of doubled haploid breeding and three systems of conventional breeding were compared for two criterion parameters, i.e., the probability of obtaining desirable genotypes and the expected genetic advance of selected lines. It was inferred that the efficiency of production of haploid and doubled haploid plants primarily determines the success of the doubled haploid breeding method. In doubled haploid breeding, about 1/5, hopefully 1/2 as many test plants need to be raised as in conventional breeding to achieve the same level of success. With this condition begin satisfied, the doubled haploid breeding method can efficiently be used when one or more of the following conditions are met: (i) a relatively small number of loci, presumably ten of less, is involved with the breeding objective concerned, (ii) desirable alleles are recessive to undesirable ones at most, if not all, of the segregating loci, and (iii) the genes are not strongly linked. It was confirmed that the doubling of haploids can better be applied to selected F2 plants rather than to F1 plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041487
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A definition of the optimal allocation of effort in conservation of plant genetic resources-with application to sample size determination for field collection (1985)
    Springer
    Euphytica 34 (1985), S. 345-354 
    Details
    ISSN: 1573-5060
    Keywords: Plant genetic resources ; conservation method ; sample size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A criterion was presented to define the most efficient strategy for the exploration and maintenance of plant genetic resources. All of the three factors composing the efficiency. i.e., multiplicity of target populations. the amount of expenses, and goodness for individual populations of the conservation manipulation adopted, were incorporated in the present criterion. Sample size per target population for field collection was investigated on the basis of this criterion, leading to the conclusion that the number of visited populations rather than sample size per population determines the overall efficiency of a collection project as a whole. Without any particular reason, intensive sampling for a limited number of populations is not logical. A sample size as small as ten plants per site or population was estimated reasonable to cover a large target area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022927
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Sample size for collecting germplasms from natural plant populations in view of the genotypic multiplicity of seed embryos borne on a single plant (1989)
    Springer
    Euphytica 41 (1989), S. 91-97 
    Details
    ISSN: 1573-5060
    Keywords: Sampling method ; sample size ; field collection ; genetic resources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method to collect germplasms from natural plant populations (collection sites) has been investigated for the numbers of plants and seeds per plant to be sampled. It is derived that in predominantly selfing populations the success of sampling is primarily determined by the number of plants rather than seeds per plant, since the genotypes of seed embryos produced on a highly selfing plant are highly homozygous and homogeneous. The number of plants, however, does not need to be large. The drawback of a shortage in the plant number can be avoided by collecting sufficient seeds from each plant. Computations for some probable situations lead to the conclusion that a few plants per population may be enough if the plants bear a few hundred seeds each and are not highly selfing. This sample size is much smaller and more practicable than those proposed previously.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022417
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...