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  • 1980-1984  (1)
  • 1975-1979  (2)
  • 1970-1974  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Composition of the proteinaceous gel secretion from the skin of the Arabian Gulf catfish (Arius thallasinus) (1982)
    Springer
    Marine biology 70 (1982), S. 27-33 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Arabian Gulf catfish Arius thallasinus excretes copious amounts of proteinaceous gel from epidermal cells when threatened or injured. As a means of investigating its biological role, this gel material has been examined to determine the nature of the secretion and its associated biological activities. Over 85% of the dry weight of the gel material is protein with small amounts of lipids, carbohydrates and nucleic acid components. Most of the protein exists as a high molecular weight aggregate of predominantly 13 500 molecular weight monomeric units. Several lytic enzyme activities are found in the gel secretion, one of which catalyzes lysis of red blood cells. Other factors induce clotting in citrate treated plasma and agglutination of red blood cells. The enzymic properties of the gel secretion resemble those of some animal venoms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397293
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular and genetic events accompanying petite induction and recovery of respiratory competence induced by ethidium bromide (1976)
    Springer
    Molecular genetics and genomics 144 (1976), S. 263-272 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The treatment of yeast cells with high levels of ethidium bromide causes a rapid induction of respiratory deficient mutants followed by a period of recovery to respiratory competence in 60 to 70% of the cells. Prolonged exposure then results in a final irreversible phase of petite formation. Sucrose gradient sedimentation analysis of 3H-adenine labelled mtDNA indicates that limited fragmentation (to about 16-18S) occurs during the initial phase of petite induction followed by a reassembly of the fragments during the period corresponding to the recovery of respiratory competence. The reassembly is associated with an ethidium bromide insensitive incorporation of 3H-adenine into mtDNA at a level consistent with repair synthesis. Genetic analyses, based on the transmission of five markers carried on the mtDNA of “repaired ρ+” clones, suggests that reassembly occurs with a high degree of fidelity, though in two of a total of twenty five clones differences in marker transmission frequency were observed which could possibly reflect an altered gene order. In addition, a description is given of the marked changes in the suppressive nature of the treated cells and the temporary reduction in the capacity for marker transmission seen to accompany the transitory fragmentation of the mtDNA. The final phase of petite induction is an energy dependent degradation of the mtDNA to produce a ρ0 culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341724
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Factors affecting petite induction and the recovery of respiratory competence in yeast cells exposed to ethidium bromide (1976)
    Springer
    Molecular genetics and genomics 144 (1976), S. 253-262 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When growing cultures of S. cerevisiae are treated with high concentrations of ethidium bromide (〉50 μg/ml), three phases of petite induction may be observed: I. the majority of cells are rapidly converted to petite, II. subsequently a large proportion of cells recover the ability to form respiratory competent clones, and III. slow, irreversible conversion of all cells to petite. The extent of recovery of respiratory competence observed is dependent on the strain of S. cerevisiae employed and the temperature and the carbon source used in the growth medium. The effects of 100 μg/ml ethidium bromide are also produced by 10 μg/ml ethidium bromide in the presence of the detergent, sodium dodecyl sulphate, and recovery is also observed when cells are treated with 10 μg/ml ethidium bromide under starvation conditions. Genetic analysis of strain differences indicates that a number of nuclear genes influence petite induction by ethidium bromide. In one strain, S288C, petite induction by 100 μg/ml ethidium bromide is extremely slow under certain conditions. Mitochondria isolated from S288C lack the ethidium bromide stimulated nuclease activity found in D243-4A, a strain which shows triphasic kinetics of petite formation. This enzyme may, therefore, be responsible for the initial phase of rapid petite formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341723
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Recombination in yeast mitochondrial DNA (1972)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 1 (1972), S. 145-152 
    Details
    ISSN: 0091-7419
    Keywords: Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: When haploid yeast strains containing mitochondrial DNAs (mtDNAs) of different buoyant densities are mated, the resulting zygotes contain a mixed population of mitochondria and mitochondrial DNAs. During vegetative growth of diploid cells formed from such a cross between a petite strain with mtDNA of density 1.677 g cm-3 and a respiratory competent strain with mtDNA of density 1.684 g cm-3, mtDNAs with intermediate buoyant densities are obtained. Virtually all newly synthesized mtDNA in diploid ρ- progeny has the intermediate buoyant density. Therefore, within 2 generations of growth of the diploid cells, the intermediate buoyant density species predominate. In crosses between a respiratory competent strain and other petite strains with different values of genetic suppressiveness, it was found that the amount of recombination yielding mtDNAs of intermediate buoyant densities roughly parallels the degree of suppressiveness. Individual clones of respiratory deficient cells from such crosses were also isolated to confirm that stable mtDNAs with intermediate buoyant densities were obtained. Thus, it is apparent that some form of recombination takes place within the mtDNAs of yeast cells that results in stable mtDNA species.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400010207
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    Paper (German National Licenses)
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