ISSN:
1432-0428
Keywords:
Crosslinking
;
disuccinimidyl suberate
;
insulin receptor
;
anti-receptor antibody
;
immunoprecipitation
;
type B syndrome
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 125I-insulin was covalently crosslinked to its receptor on human placental membrane fractions with disuccinimidyl suberate. The 125I-insulin crosslinked receptor was solubilized with Triton X-100 and used as a probe to determine autoantibodies to the insulin receptor in sera from patients with insulin resistance (type B syndrome). When the solubilized 125I-insulin: receptor complex was incubated with these sera and then with anti-human IgG serum, the complex was precipitated as a function of the amount of anti-receptor serum. Unlabelled insulin at concentrations ⩽10 μg/ml did not affect immunoprecipitation of the complex, suggesting that a subpopulation of anti-receptor antibodies recognizes determinants outside the insulin binding region of the receptor molecule. 125I-insulin: receptor complex was precipitated also by the addition of anti-insulin antibodies. The effect of anti-insulin antibody was eliminated by the addition of excess unlabelled insulin. The immunoprecipitation assay using 125I-insulin crosslinked receptor was sensitive and convenient for detecting anti-receptor antibodies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00251830
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