Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1980-1984  (4)
  • 1975-1979  (1)
Source
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    In Vitro Cultivation of Bloodstream Forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . A series of new in vitro systems for the cultivation of bloodstream forms of Trypanosoma (Trypanozoon) brucei brucei, T. (T.) b. rhodesiense, and T. (T.) b. gambiense was developed. The standard system consists of a feeder layer of fibroblast-like cells derived from embryos of New Zealand White rabbits (REF) or a mountain vole, Microtus montanus (MEF), with HEPES-buffered Minimum Essential Medium (MEM), with Earle's salts, supplemented with 15% inactivated rabbit serum. These two and other feeder layers were cross-checked with different sera to test for growth support of bloodstream forms of the three trypanosome subspecies studied. Cultures could be initiated with bloodstream forms from mammalian hosts or from cryopreserved stabilates. Metacyclic forms from infected Glossina m. morsitans could also be used as inoculum; they transformed within 6 h to bloodstream forms. Maintenance of cultures and growth properties are described in detail. Experiments were undertaken to confirm that the cultivated bloodstream forms still possess some of the characteristic features of pleomorphic bloodstream populations. Cultivated bloodstream forms were always infective for mice, and a surface coat could be demonstrated by electron microscopy. They could also be cyclically transmitted through tsetse flies, and the metacyclic forms from these flies could be brought back into culture. In vitro cloning with single bloodstream forms and metacyclic forms could be achieved with high cloning efficiency. The consumption of glucose and the production of pyruvate and lactate were determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb05322.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Oocyte maturation in vitro: Contribution of the oviduct to total maturation inXenopus laevis (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 1275-1276 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Contact of progesterone matured oocytes ofXenopus laevis with the oviduct reduces the time necessary to attain cleavage capacity from 24 h to 3 h. Full maturity has been demonstrated by normal development of the matured eggs after fertilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01945777
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cyclical transmission of in vitro cultivated bloodstream forms and procyclic trypomastigotes ofTrypanosoma brucei brucei byGlossina morsitans morsitans (1982)
    Springer
    Parasitology research 68 (1982), S. 1-5 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In vitro cultivated bloodstream and procyclic forms ofTrypanosoma b. brucei STIB 247 were cyclically transmitted byGlossina m. morsitans. The tsetse flies were infected artificially on a silicon membrane. Metacyclic trypanosomes from mature salivary gland infections were used to initiate bloodstream form cultures. They transformed into slender bloodstream forms and gave rise to established cultures that proved to be infective for the vector. The metacyclic forms retained the strain-specific basic set of variable antigen types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00926651
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Stimulating effect of citrate and cis-aconitate on the transformation ofTrypanosoma brucei bloodstream forms to procyclic forms in vitro (1981)
    Springer
    Parasitology research 66 (1981), S. 17-24 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Transformation ofTrypanosoma brucei bloodstream forms to procyclic forms can be stimulated in vitro by adding 2–10 mM citrate and/or cis-aconitate to the culture medium for at least 3 h. Optimal transformation was obtained in the presence of 3 mM cis-aconitate for 48 h. Citrate and cis-aconitate may activate isocitrate dehydrogenase and/or other Krebs' cycle enzymes and thus act as a metabolic trigger for the morphological changes involved in transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00941941
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Cultivation of procyclic trypomastigotes ofTrypanosoma congolense in a semi-defined medium with direct adaptation from bloodstream forms (1982)
    Springer
    Parasitology research 67 (1982), S. 129-135 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Bloodstream forms ofTrypanosoma congolense added to the semidefined medium 109-c (Minimum Essential Medium with Earle's salts, 60 mM HEPES, haemin, and heat-inactivated FBS) and incubated at 28° C transformed into procyclic trypomastigotes. Established cultures of the procyclic forms yielded up to 4.5×107 parasites/ml. The cultivated populations consisted only of trypomastigotes and were not infective for mice. The amino acids consumed to the greatest extent were glutamine, proline, and threonine. Changes in glucose, pyruvate, and lactate concentrations during growth were determined. The procyclic organisms possessed a well-developed mitochondrion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00928108
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...