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  • 1980-1984  (2)
  • 1970-1974  (3)
  • 1960-1964  (1)
  • Chemistry  (4)
  • Hymenoptera
  • flow cytometry
  • 1
    Electronic Resource
    Electronic Resource
    Nerol: An alarm substance of the stingless bee,Trigona fulviventris (Hymenoptera: Apidae) (1982)
    Springer
    Journal of chemical ecology 8 (1982), S. 1167-1181 
    Details
    ISSN: 1573-1561
    Keywords: Alarm substance ; nest defense ; nerol ; mandibular gland ; Hymenoptera ; Apidae ; Meliponinae ; stingless bees ; Trigona fulviventris ; Apiomerus pictipes ; Hemiptera ; Reduviidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bees of the genusTrigona and subgenusTrigona possess volatile materials in their mandibular glands, used as alarm substances and as marking pheromones. Heads of workers ofTrigona fulviventris were analyzed by gas chromatography-mass spectrometry. The two major volatile components were nerol (∼ 50%), and octyl caproate (∼ 20%). Relative to other substances tested at a Costa Rican nest, treatments containing 20 μg of nerol attractedT. fulviventris, depressed numbers of bees leaving the nest by about 50%, and elicited wing vibration and biting. The responses were similar to those obtained with the contents of one worker head. Attraction and biting were also seen in response to captures of colony members by assassin bugs (Apiomerus pictipes) outside a nest entrance; one bee responded in about 15% of the captures. This alarm behavior, although weak, is of interest since it was thought thatT. fulviventris was unusual for its subgenus in its lack of nest defense behaviors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00990750
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  • 2
    Electronic Resource
    Electronic Resource
    Zur Bestimmung der Kettenlänge in isoprenoid substituierten Chinonen (1960)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 43 (1960), S. 2088-2089 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The number of isoprene units in quinones with isoprenoid side chains is determined by quantitative NMR-spectrometry.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19600430726
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  • 3
    Electronic Resource
    Electronic Resource
    Ag-Sn-Hg alloys as electron compounds (1970)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 4 (1970), S. 269-274 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The extraordinary stability of the γ1 phase of dental amalgam was believed to indicate that part of the Sn in dental amalgam was contained in this phase. By extending the Hume-Rothery “electron compound” ideas to the three component Ag-Sn-Hg system, an estimate was made of the amount of Sn which could be held in the γ1 structure.A Ag-Sn alloy containing 9.1 weight per cent Sn was prepared, spheroidized, and sized. Portions were amalgamated and molded into tensile specimens, parts of which were subjected to X-ray diffraction, corrosion tests, and tests to determine amalgamation rate and dimensional changes during setting.X-ray diffraction patterns showed a complete absence of the γ2 phase, (thus indicating that all Sn was in the γ1 phase) and a stability intermediate between γ Ag-Hg and the γ1 phase of dental amalgam. Physical tests showed a low amalgamation rate, low 1-hr strength, high 7-day strength, and high corrosion resistance.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820040207
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  • 4
    Electronic Resource
    Electronic Resource
    The amount of tin in the γ1 phase of dental amalgam (1971)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 5 (1971), S. 239-244 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Spherical-particle Ag-Sn alloy powders containing 4.3, 8.3, 12.0, 15.4, 18.5, 21.4, and 24.1 atomic per cent Sn were obtained. Amalgams from these alloys were examined by x-ray diffraction and metallography for the presence of the γ2 phase and the γ1 → β1 phase transformation. A single amalgam of the approximate composition of dental amalgam was prepared from a melt at high temperature and pressure and likewise examined.Results showed that the solubility of Sn in Ag-Hg is about 7 atoms of Sn per unit cell of the γ phase. As clinically prepared, the γ1 phase of dental amalgam contains, however, only about 5 atoms of Sn per unit cell. These 5 atoms, nevertheless, result in a very considerable stabilization of this phase due to the increase in its electron concentration.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820050310
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  • 5
    Electronic Resource
    Electronic Resource
    Splat-cooled alloys in the Ag-Sn-Cu system (1974)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 8 (1974), S. 3-10 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Strength, dimensional change during setting, and corrosion resistance have been measured for amalgams prepared from splat-cooled alloys of different compositions in the Ag-Sn-Cu system. The amalgam with optimum values of these properties was made from the alloy containing 60 at. % Ag, 25 at. % Sn, and 15 at. % Cu. Tensile strengths for amalgams from this alloy were about 8000 psi, dimensional change during setting near zero, and corrosion resistance was good.X-Ray diffraction showed that the γ1 and γ2 phases formed during the early stages of setting but that the γ2 phase had essentially disappeared after about 24 hr. At the latter time Cu6Sn5 was present.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820080103
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  • 6
    Electronic Resource
    Electronic Resource
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100402
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