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Mast cell numbers in incisional wounds in rat skin as a function of distance, time and treatment (1983)

PERSINGER, M.A. ; LEPAGE, PATRICIA ; SIMARD, J.-P. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

British journal of dermatology 108 (1983), S. 0

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ISSN: 1365-2133

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: The dynamic changes in skin mast cell (MC) numbers around incised wounds were studied, using experimental designs amenable to multiple analyses of variance. Sixty-four Wistar albino rats were shaved in the interscapular region, wounded or not wounded, and then killed 2 or ro days later. During this period, the rats were exposed continually to a cold (2° C) or control (20° C) climate and treated daily over the shaved region with either tap water or a weak sulphuric acid (pH 3.5) solution. The MCs within five adjacent fields of the wound or the control reference and within the superficial and deep halves of the skin were counted (at × 400). The greatest decrease in MC numbers occurred within about 700 μm of the wound. Whereas the paucity of MCs within the wound region was evident at 2 days, near-normal levels were achieved by day 10. Cold exposure produced little effect, but MCs responded differently to the water and acid treatments as a function of distance and skin depth.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2133.1983.tb00060.x

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L -Asparaginase and Complement (1971)

DELAGE, J. M. ; SIMARD, J. ; LEHNER-NETSCH, G. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 233 (1971), S. 485-486

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Human-serum (1 ml.) was diluted five times and incubated for 2 h at 22 C with 50 U of L-asparaginase (LA). The enzyme was obtained from Merck, Sharpe and Dohme and was dialysed overnight against phosphate buffer (0.005 M) before use. The serum was then diluted a further ten times and its complement ...