ISSN:
1432-0878
Schlagwort(e):
Melanotrophs
;
Immuno-gold technique
;
Secretory vesicles
;
γ 3-MSH
;
α-MSH
;
Acetylation
;
Rabbit
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
Notizen:
Summary Melanotrophic cells of the pars intermedia of the rabbit were studied at the electron-microscopic level by means of the immuno-gold technique with the use of antisera against γ 3-MSH and α-MSH. Both antibodies labelled all secretory vesicles stored in the peripheral cytoplasm, but secretory vesicles and intrasaccular condensations in the Golgi area reacting for γ 3-MSH were not labelled with the antibody against α-MSH. Since this antibody appears to recognise only the acetylated form of α-MSH, the present observations suggest that acetylation occurs at a stage subsequent to the Golgi packaging, during maturation of the secretory vesicles. Thus, the morphological evidence supports biochemical data in favour of intragranular processing of opiomelanocortin in melanotrophic cells.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00216197
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