ISSN:
1432-2048
Keywords:
ATPase
;
Crassulacean acid metabolism
;
Kalanchoë
;
Protoplast lysis (polybaseinduced)
;
Vacuole (ATPase)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A technique is described that allows a relatively rapid and controlled isolation of vacuoles from leaves of the crassulacean acid metabolism (CAM) plant Kalanchoë daigremontiana. The method involves polybase-induced lysis of mesophyllcell protoplasts and isolation of vacuoles on a discontinuous density gradient. ATPase activity is associated with the isolated vacuoles and is not attributable to contamination by cytoplasmic constituents. It is suggested that this ATPase is responsible for the energization of malic-acid accumulation in the vacuole in CAM plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00396740
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