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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Behavior genetics 12 (1982), S. 111-121 
    ISSN: 1573-3297
    Keywords: neurotransmitters ; genetics ; quantitative genetics ; pharmacology ; environment ; genetic correlations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Genetic studies of neurotransmitter processes to date differ from most behavioral genetic studies in that the former have rarely considered individual variability, have tended to look only for single-gene effects, and generally have overlooked or ignored environmental influences. After examining these differences, we suggest that a rapprochement between the two fields will yield synergistic benefits in studies of the genetics of neuropharmacological processes as they affect behavior.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Chalcone synthase ; Colletotrichum ; Elicitor ; Phaseolus ; Phenylalanine ammonia-lyase ; Phytoalexin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In wounded cotyledons ofPhaseolus vulgaris L. the accumulation of the 5-hydroxy isoflavonoids kievitone and 2′-hydroxygenistein precedes the major increases in the levels of the 5-deoxy compounds phaseollin and coumestrol. Increased phytoalexin levels are preceded by transient increases in the extractable activities of L-phenylalanine ammonia-lyase (EC 4.3.1.5.), chalcone synthase and chalcone isomerase (EC 5.5.1.6.). Accumulation of phytoalexins, above wounded control levels, is observed following treatment of excised cotyledons or hypocotyls with crude or fractionated elicitor preparations heat-released from the cell walls ofColletotrichum lindemuthianum. Chalcone synthase levels are also induced in cotyledons, although crude elicitor and all fractions suppress L-phenylalanine ammonia-lyase activity in both tissues. Kievitone is the major phytoalexin induced in cotyledons, whereas in hypocotyls phaseollin predominates. Patterns of phytoalexin accumulation have been studied in response to varying concentrations of the crude and fractionated elicitor; 5-hydroxy isoflavonoid accumulation is highly dependent upon elicitor concentration, the dose-response curves for kievitone accumulation showing maxima at around 1 μg glucose equivalents per cotyledon, minima at 2–3 μg equivalents and increasing induction at higher concentrations. Similar patterns are observed for L-phenylalanine ammonia-lyase and chalcone synthase levels, although the overall extent of these changes is masked by the high wound response. Accumulation of 5-deoxy isoflavonoids above control levels requires high elicitor concentrations; no experimental conditions were found under which phaseollin accumulated to higher levels than kievitone in cotyledons during the first 48 h after elicitation.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Cell suspension culture ; Chalcone isomerase ; Elicitor ; Enzyme induction ; Phaseolus (chalcone isomerase) ; Phytoalexin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Approximately fourfold increases in the extractable activity of the enzyme chalcone isomerase (CHI, EC 5.5.1.6) were observed within 24 h of treatment of cell suspension cultures of Phaseolus vulgaris with a crude elicitor preparation heatreleased from the cell walls of the bean pathogen Colletotrichum lindemuthianum. The induction of CHI activity was highly dependent upon elicitor concentration, with maximum induction occurring in two discrete concentration ranges. A basal half-life for CHI〉32 h in control cultures was determined by labelling with 2H from 2H2O followed by analysis of the equilibrium distribution of enzyme activity in CsCl density gradients. Comparative density labelling indicated that at both the lower and higher effective elicitor concentrations, the induced appearance of CHI activity was the result of an apparent initial activation of pre-existing enzyme followed by an increase in the rate of de-novo synthesis of the enzyme as compared with non-elicited controls. The increased appearance of the enzyme over the first 8 h in elicitor-treated cultures was inhibited by cycloheximide, cordycepin and actinomycin D. The results are discussed in relation to the mechanisms of co-ordinate enzyme induction operating in French-bean cell cultures exposed to fungal elicitors.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: Pelargonium × hortorum ; Pelargonium ; fertilization ; embryo survival ; plastid crosses ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The 6×6×4 analysis of variance of the mean percentage embryo survival for six variegated cultivars of Pelargonium × hortorum Bailey, with mutant plastids in their germ layers, and for their isogenic green (G) clones, shows highly significant differences between females (81 to 91 per cent) and between plastid crosses (83 to 91 per cent). Between cultivar differences are attributed largely to additive effects corresponding to additive gene action, and between plastid differences to a lower survival after crosses with white (W) males than with green males. The relationship between overall fertility (% fertilization × survival) and plastid crosses is a stepwise decline in the order G×G〉G×W〉W×G〉W×W (28 to 19 per cent) in which the white embryos growing in a white mother are approximately 30 per cent less fertile than the green embryos in a green mother. The non-surviving embryos are classified into empty, undeveloped and dwarf embryos and submitted to a 6×4 analysis of variance after summing through males. The significant heterogeneity between females is attributed largely to a difference between one cultivar-with a high frequency of dwarf and a low frequency of undeveloped embryos-and the other five cultivars. The absence of a significant plastid effect upon any stage of embryo breakdown indicates that the depressive effect of white plastids is spread evenly throughout development.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 33 (1984), S. 169-176 
    ISSN: 1573-5060
    Keywords: Mirabilis jalapa ; marvel of Peru ; four o'clock ; leaf variegation ; mutable genes ; controlling elements ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The recurrent back-mutation from homozygous recessive chlorina to heterozygous dominant green in leaves of variegated Mirabilis jalapa is explicable on the hypothesis of a controlling element system. The analysis of variance of mutation frequency suggests that there is polygenic control modifying the major regulatory gene, an idea which is supported by a significant parent offspring regression. There is also evidence for a change of state in the regulatory element giving rise to a new mutability pattern.
    Type of Medium: Electronic Resource
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