ISSN:
1432-072X
Schlagwort(e):
Phanerochaete chrysosporium
;
Lignin degradation
;
Veratryl alcohol
;
Secondary metabolism
;
Mutants
;
Phenol oxidase
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A pleiotropic mutant of Phanerochaete chrysosporium 104-2 lacking phenol oxidase and unable to form fruit bodies and a revertant strain 424-2 were isolated after UV mutagenesis. Strains 104-2 and 424-2 had no apparent dysfunction in primary metabolism with glucose as a carbon source. Unlike the wild type strain and strain 424-2, strain 104-2 was unable to evolve 14CO2 from 14C ring, side chain and 3-O-14C-methoxy labeled lignin. In addition, strain 104-2 was unable to evolve 14CO2 from a variety of lignin model compounds including 14C-4′-methoxy labeled veratrylglycerol-β-guaiacyl (V) ether, γ-14C-guaiacylglycerol-β-guaiacyl ether (VI), as well as 1-(14C-4′-methoxy, 3′-methoxyphenyl)1,2 propene (III) and 1-(14C-4′-methoxy-3′-methoxyphenyl) 1,2 dihydroxypropane (IV). The addition of peroxidase/H2O2 to cultures of strain 104-2 did not alter its capacity to degrade the labeled lignins. A variety of unlabeled lignin model compounds previously shown to be degraded by the wild type organism including β-aryl ether dimers and diaryl propane dimers were also not degraded by the mutant 104-2. The revertant strain 424-2 regained the capacity to degrade these compounds. The substrates described are degraded by oxygen requiring system(s) expressed during the secondary phase of growth, suggesting this pleiotropic mutant is possibly defective in the onset of postprimary metabolism. The inability of the mutant to produce the secondary metabolite veratryl alcohol and to elaborate enzymes in the veratryl alcohol biosynthetic pathway supports this hypothesis.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00508715
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