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15N nuclear magnetic resonance studies of [1-15N]nicotinamide adenine dinucleotides (1980)

Oppenheimer, Norman J. ; Davidson, Robert M.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 13 (1980), S. 14-16

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ISSN: 0030-4921

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The synthesis of [1-15N]nicotinamide adenine dinucleotide is described. Chemical shift data from 15N NMR studies are presented for the pyridine ring nitrogen of labeled NAD and related compounds. The results indicate a 15N label in the N-1 position to be highly sensitive to the redox-state of the pyridine moiety, with an upfield shift of over 100 ppm observed upon reduction of NAD+ to NADH. The feasibility of conducting 15N NMR studies of pyridine nucleotide binding to dehydrogenases is discussed.

Additional Material: 1 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/mrc.1270130104

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