ISSN:
1432-2048
Keywords:
Avena (phytochrome)
;
Immunological discrimination
;
Monoclonal antibody
;
Phytochrome (red-, far-red absorbing forms)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00393420
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