ISSN:
1432-0983
Keywords:
dTMP starvation
;
Sister chromatid recombination
;
rad mutants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Inhibition of thymidylate biosynthesis has been found to induce deletion of a LEU2 insert from the ribosomal DNA gene cluster of haploid strains of Saccharomyces cerevisiae. Loss of the insert was detected phenotypically by the enhanced production of both sectored (leu+/leu−) and non-sectored (leu−) colonies. Hybridization patterns obtained by Southern blot analysis of DNA from the leu+ and leu− sectors were consistent with the occurrence of unequal sister chromatid recombination. The induction of sectored colonies was prevented by the rad52-1 mutation but not by defects in RAD6. However, the formation of non-sectored leu− colonies was induced by thymidylate depletion in both rad52-1 and rad6 strains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00417818
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