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Quantitative ion localization within Suaeda maritima leaf mesophyll cells (1981)

Harvey, Diana M. R. ; Hall, J. L. ; Flowers, T. J. ; [et al.]

Springer

Planta 151 (1981), S. 555-560

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ISSN: 1432-2048

Keywords: Halophyte ; Ion accumulation ; Suaeda

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00387435

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An application of the INEPT pulse sequence to the NMR assignment of halogenated marine natural products (1983)

Brownlee, R. T. C. ; Hall, J. G. ; Reiss, J. A.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 21 (1983), S. 544-547

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ISSN: 0030-4921

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The routine use of the INEPT pulse sequence is shown to be the method of choice for the assignment of multiplicity from 13C NMR spectra where no prior information about the 1J(CH) coupling constants is available, or where these are expected to vary over a large range. This technique is illustrated using spectra obtained from polyhalogenated monoterpenes isolated from the red alga Plocamium angustum, which have 1J(CH) values varying from 127 to 203 Hz.

Additional Material: 3 Ill.