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  • 1980-1984  (4)
  • Autotransfusion  (2)
  • Transmitter release  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Mitochondrial release of Ca2+ during sustained nerve activity in the electric organ of Torpedo marmorata (1980)
    Springer
    Experimental brain research 38 (1980), S. 405-417 
    Details
    ISSN: 1432-1106
    Keywords: Calcium ; Mitochondria ; Cholinergic nerve ending ; Torpedo ; Transmitter release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The calcium content of mitochondria isolated from the Torpedo electric organ was determined by atomic absorption spectroscopy. The mitochondrial fraction was found to contain 830 ng-atom Ca/mg protein. For comparison a fraction of isolated nerve endings contained 250 ng-atom Ca/mg protein. When tissue was continuously stimulated at a frequency of 5 Hz via the electromotor nerves, the Ca content of the mitochondrial fraction which is likely to contain both pre- and postsynaptic mitochondria was reduced proportionally to the decrease in transmitter content of the isolated nerve terminal fraction. After 5,000 pulses, the mitochondrial fraction had lost 80% of its Ca contents. When tissue was stimulated after previous perfusion with d-tubocurarine and blockade of electrical response the same number of pulses caused only a loss of 20–40% in the Ca content of the mitochondrial fraction. Release of transmitter was not affected by d-tubocurarine. The results suggest that repetitive stimulation in the absence of postsynaptic receptor blockade causes loss of Ca from both nerve terminal mitochondria and mitochondria of the myotube-derived electrocytes. In the presence of d-tubocurarine Ca is lost from presynaptic mitochondria only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237520
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Preferential calcium staining of mitochondria in stimulated cholinergic nerve endings (1980)
    Springer
    Experimental brain research 38 (1980), S. 419-424 
    Details
    ISSN: 1432-1106
    Keywords: Mitochondria ; Calcium staining ; Cholinergic nerve ending ; Torpedo ; Transmitter release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The fine structure of the Torpedo electric organ was investigated during different functional states using fixation media with elevated calcium concentrations. When electric tissue was stimulated (5,000 pulses, 5 Hz) and subsequently fixed in an s-collidine buffered aldehyde fixative containing 5 mM CaCl2 mitochondria in both nerve terminals and postsynaptic electroplaque cells showed electron-dense deposits. When tissue blocks were stimulated in the presence of d-tubocurarine (250 μM) which blocked electrical response deposits were restricted to presynaptic mitochondria. Electron-dense deposits were never observed in unstimulated tissue. The observations suggest that after stimulation mitochondria have an increased calcium binding capacity. In conjunction with previous biochemical findings (Schmidt and Zimmermann, 1980), we suggest that this increased binding capacity is due to a previous stimulation induced loss of calcium from activated mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237521
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Intraoperative autotransfusion (1982)
    Springer
    Langenbeck's archives of surgery 358 (1982), S. 556-556 
    Details
    ISSN: 1435-2451
    Keywords: Autotransfusion ; Range of use ; Results ; Autotransfusion ; Verwendungsmöglichkeiten ; Ergebnisse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An der Chirurgischen Universitätsklinik Köln wurde ein neues Autotransfusionssystem bei 14 Patienten 56mal eingesetzt (Solcotrans). Dieses System besteht aus einem Kunststoffbehälter, der über einen Luftschlauch mit einer Vacuumpumpe verbunden wird. Es können mit diesem Einmalgerät vom Chirurgen 500ml Blut aufgesaugt und durch den Anaesthesisten wieder reinfundiert werden. Das System wurde bei venösen Thrombektomien, Bauchaortenaneurysmen und in der Shuntchirurgie bei portaler Hypertension eingesetzt. Bei 9 Operationen konnte dadurch auf eine Fremdblutzugabe verzichtet werden.
    Notes: Summary At the surgical unit of the University of Cologne a new system for autotransfusion (Solcotrans) was used 56 times in 14 patients. This system consists of a plastic case that is connected with a vacuum pump by an air tube. After filling the plastic case with 30 ml of sodium citrate, 500 ml of blood can be aspirated by the surgeon and reinfused by the anaesthetist. The system was used in venous thrombectomy, aneurysm of the aorta abdominalis and in shunt surgery by portal hypertension. Because of this system donor blood was not used in nine operations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01272002
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    193. Ein neues Verfahren zur intraoperativen Autotransfusion (1984)
    Springer
    Langenbeck's archives of surgery 364 (1984), S. 525-525 
    Details
    ISSN: 1435-2451
    Keywords: Intraoperative autotransfusion ; Application ; Results ; Autotransfusion ; Anwendung ; Ergebnisse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An der Chirurgischen Universitäts-Klinik Köln-Lindenthal wurde ein neues System zur intraoperativen Autotransfusion bei 43 Patienten eingesetzt. Dieses System besteht aus einem stabilen Kunststoffbehälter, der einen flexiblen Kunststoffbeutel enthält. Nach Aspiration von 70 ml Natrium-Citrat-Lösung können bis zu 500 ml Blut aufgesaugt und durch den Anaesthesisten unter Druck reinfundiert werden. Bei 25 Operationen konnte dadurch auf eine Fremdblutgabe verzichtet werden. Das System eignet sich zum Einsatz in der Gefäß-, Abdominal- und Unfallchirurgie. Blutqualitätskontrollen wurden durchgeführt.
    Notes: Summary In the surgical unit of the University of Cologne a new system for intraoperative autotransfusion has been used in 43 patients. This system consists of a rigid plastic case with a plastic bag inside. After filling the plastic bag with 70 ml of natrium citrate solution, 500 ml of blood can be aspirated by the surgeon and reinfused under pressure by the anesthetist. Through this stored blood was not necessary in 25 operations, the set is well suited for intraoperative autotransfusion in vascular and abdominal surgery and in cases of trauma. Blood quality examinations were carried out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01823367
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    Paper (German National Licenses)
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