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  • 1
    ISSN: 0942-0940
    Keywords: Brain tumours ; LDH isoenzyme ; cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The lactate dehydrogenase (LDH) isoenzyme patterns in benign and malignant brain tumours were determined by means of electrophoresis of the cell extracts and selective cytochemical stain of the smears. The LDH isoenzyme distribution of the cell extracts showed a pronounced cathodal shift in the malignant gliomas and metastatic carcinomas. Normal brain tissues and histologically benign gliomas, however, showed an anodal pattern with a dominance of the H-type LDH. Schwannomas and meningiomas had a midzone isoenzyme pattern with a dominant LDH3 fraction. Pituitary adenomas usually showed the LDH pattern similar to that of the normal cerebrum. The LDH M fraction could be cytochemically verified using an inhibitory effect by 2.6 M urea in staining. Astrocytomas grades 3–4 and metastatic carcinomas were characterized by loss or marked reduction of stainability by urea treatment, while astrocytomas grades 1–2 and oligodendrogliomas were resistant to urea inhibition.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 19 (1981), S. 340-348 
    ISSN: 1741-0444
    Keywords: Gas exchange ; Impedance plethysmography ; Microcomputer ; Online measurement ; Ventilation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A microcomputer-based system for the breath-by-breath assessment of respiratory and metabolic function during exercise was developed. To minimise the load for subjects ventilation was measured by use of an impedance plethysmograph instead of a pneumotachograph. The computer calculated respiratory frequency, tidal volume, minute ventilation, end-tidal pO2 and pCO2, O2 input and CO2 output, and gas exchange ratio of each breath from the impedance pneumogram and the gas composition of the expired air. To avoid the error due to the cardiogenic oscillations appearing to overlap the impedance pneumogram, discrimination of the inspiratory and expiratory phases was achieved by detecting the temperature difference between ambient and expired air. The impedance pneumogram could follow tidal volume variations during a moderate bicycle exercise (120 W) with satisfactory accuracy, but its sensitivity is apt to fall at the severe exercise (180 W). Possible factors causing errors such as the distribution of regional ventilation and perfusion during exercise are discussed.
    Type of Medium: Electronic Resource
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