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    ISSN: 1435-2451
    Keywords: Gastrointestinal tumor cells ; Flow cytometry ; Carcinoembryonic antigen ; Epithelial membrane antigen ; Gastrointestinale Tumorzellen ; Durchflu¢ytometrie ; Carcinoembryonales Antigen ; Epitheliales Membranantigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Einzelzellsuspensionen gastrointestinaler Carcinome bzw. Mucosa wurden fluorescenzmarkiert zur simultanen Bestimmung von carcinoembryonalem (CEA) bzw. epithelialem Membranantigen (EMA) und DNA-Gehalt. Die Messung im Fluvo-Metricell-Durchflußcytometer ergab bei den Carcinomen zwischen 1% und 30% CEA- bzw. EMA-positive Zellen. Ausgeprägte Antigenmarkierung fand sich in der GO/GlZellcyclusphase, seltener bei höherploiden Populationen. Die Mucosa war bei '/3 der Patienten negativ oder schwach markiert, bei '/, mäßig bis nahezu gleich stark positiv wie der Turmor.
    Notes: Summary Singe-cell suspensions of gastrointestinal carcinoma and mucosa were stained with fluorescent dyes for determination of carcinoembryonic antigen (CEA) and epithelial membrane antigen (EMA). According to measurements in a Fluvo-Metricell flow cytometer and computerized data analysis the carcinomas contained between 1% and 30% of CEA- and EMA-positive cells. In the GO/Gl cell cycle phase there was a substantial antigen presence; this was rare in populations with higher degrees of chromosome set multiplication. In twothirds of the patients the mucosa was negative or only slightly stained, while in the remaining third it was stained to a moderate degree or almost as intensely as the tumor.
    Type of Medium: Electronic Resource
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