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Androgenic response from cultured anthers of a leguminous tree,Cassia siamea Lam. (1983)

Gharyal, Paramjit K. ; Rashid, A. ; Maheshwari, S. C.

Springer

Protoplasma 118 (1983), S. 91-93

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ISSN: 1615-6102

Keywords: Androgenesis ; Callus ; Cassia siamea ; Pollen embryoids

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Anthers ofCassia siamea, on culture on B5 medium supplemented with coconut milk (15%, v/v), 2,4-D (2 mg/l), and kinetin (0.5 mg/l), split open after one to two weeks of inoculation and eject a callus mass. Microscopic examination of the anthers cultured at the late uninucleate or early bi-celled stages, after 7–14 days of culture, revealed many multicellular structures at various stages of development, thus indicating the pollen origin of callus. Callus cells also showed the haploid chromosome number (n=14).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01284750

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