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Regulation of swelling of etiolated-wheat-leaf protoplasts by phytochrome and gibberellic acid (1983)

Blakeley, S. D. ; Thomas, B. ; Hall, J. L. ; [et al.]

Springer

Planta 158 (1983), S. 416-421

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ISSN: 1432-2048

Keywords: Gibberellin and protoplast swelling ; Phytochrome ; Protoplast (swelling) ; Triticum (protoplast swelling)

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4°C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m-2s-1 was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00397734

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An application of the INEPT pulse sequence to the NMR assignment of halogenated marine natural products (1983)

Brownlee, R. T. C. ; Hall, J. G. ; Reiss, J. A.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 21 (1983), S. 544-547

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ISSN: 0030-4921

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The routine use of the INEPT pulse sequence is shown to be the method of choice for the assignment of multiplicity from 13C NMR spectra where no prior information about the 1J(CH) coupling constants is available, or where these are expected to vary over a large range. This technique is illustrated using spectra obtained from polyhalogenated monoterpenes isolated from the red alga Plocamium angustum, which have 1J(CH) values varying from 127 to 203 Hz.

Additional Material: 3 Ill.