Electronic Resource
Springer
Pflügers Archiv
399 (1983), S. 83-86
ISSN:
1432-2013
Keywords:
Piezoelectric translator
;
Microelectrode
;
Intracellular electrodes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A device is described that is capable of rapidly moving microelectrodes and micropipettes over distances up to 15 μ. This piezoelectric translator uses the diaphragm from virtually any available piezoelectric buzzer in combination with simple physical support and drive electronics. All of the necessary details for the construction of this small device are presented. Each finished unit is about 2 cm long with a diameter of 2 cm and can be readily adapted to existing manipulators. The translator has been found useful in aiding the independent penetration by one or more microelectrodes of single cells or of more complicated multicellular preparations (including those that lie behind a connective tissue layer). This new device offers fine control of microelectrode motion that cannot be obtained by the other methods used to aid microelectrode and micropipette penetration of cell membranes (e.g. capacitance overcompensation — ”ringing in” or “tickling” — or tapping the manipulator base). Finally, the device described in this paper is extremely simple and inexpensive to build.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00652527
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