ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The phosphorylatable light chain of myosin (P-light chain) from hearts of the turtle, rat, frog, dog, cat, adult chicken and 1 week old chick was compared by two dimensional gel electrophoresis. The technique separates the phospho and dephospho forms of P-light chain and allows quantitation of its percentage distribution. The P-light chain was found to exist in multiple forms. The turtle heart possessed the highest percentage, 75%, of the phospho form of P-light chain, whereas 25% of P-light chain was in the dephospho form. In rat heart, 35% of the P-light chain was phosphorylated and 65% dephosphorylated. In frog heart, the P-light chain exhibited three forms: two phosphorylated and one dephosphorylated. The P-light chain of dog, cat and adult chicken hearts displayed four spots on two-dimensional gel electrophoresis: two phospho and two dephospho forms. In these species the percentage of total P-light chain being in the phospho forms was in the range of 10-25%. Comparison of adult chicken and 1 week old chick preparations suggests that during maturation from the chick to the chicken the percentage phospho form of the total P-light chain decreases. The myosin light chain kinase and phosphatase activities in whole homogenates of the above heart preparations were assayed. The data, with the exception of those from frog heart, suggest that the extent of light chain phosphorylation is correlated with the ratio of light chain kinase to phosphatase activity.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150040207
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