ISSN:
1432-0428
Keywords:
Obese-hyperglycaemic
;
obesity
;
genetic
;
mice
;
fatty acid synthesis
;
3H discrimination
;
substrates
;
lactate
;
glucose
;
ob/ob
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Fatty acid synthesis was measured in vitro in pieces of adipose tissue from lean and obese-hyperglycaemic (ob/ob) mice, using 14C-glucose or 14C-lactate and 3H2O to obtain absolute rates of total fatty acid synthesis. In the presence of lipoproteintriglyceride (2.5 μmol/l) metabolic interaction occurred which decreased glucose incorporation into fatty acids by 30% in lean mouse tissue, but not in obese mouse tissue. In the absence of added insulin, the contribution of glucose to total fatty acid synthesis was 69% in obese mouse tissue, significantly lower than the value of 87% obtained in lean mouse tissue. Insulin increased the contribution of glucose to total synthesis in both lean and obese mouse tissues, although the value in obese mouse tissue (83%) remained lower than the value in lean mouse tissue (100%). Lactate was not a major precursor for fatty acid synthesis. When both lactate (2 mmol/l) and glucose (15 mmol/l) were present, the contribution of lactate to total fatty acid synthesis was not increased in obese mouse tissue, suggesting that even in the presence of insulin, about 30% of the carbon was provided by intracellular precursors.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00261708
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