ISSN:
1749-6632
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Natural Sciences in General
Notes:
X-ray and neutron diffraction studios of oriented multilayers of isolated light sarcoplasmic reticulum (SR) have provided the separate profile structures of the lipid bilayer and the Ca2+-ATPase molecule within the membrane profile to ˜10 Å resolution. These studies utilized biosynthetically deuterated SR phospholipids incorporated isomorphously into the isolated SR membranes via exchange proteins.Time-resolved x-ray diffraction studies of these oriented SR membrane multilayers have indicated that significant changes occur in the membrane profile structure within a single turnover of the Ca2+-transport cycle. These studies utilized the flash photolysis of caged ATP to effectively synchronize the ensemble of Ca2+-ATPase molecules in the multilayer, synchrotron x-radiation to provide 100- to 500-millisecond data collection times, and double-beam spectrophotometry to monitor Ca2+ transport in the oriented SR membrane multilayer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1749-6632.1982.tb25770.x
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