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  • 1980-1984  (7)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Extraintestinal Development of Caryospora simplex (Apicomplexa: Eimeriidae) in Experimentally Infected Mice, Mus musculus (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Developmental stages of Caryospora simplex were found in connective tissue of the cheek, tongue, and nose of Swiss-Webster and C57 BL/6 mice (Mus musculus) from 8 through 70 days after oral inoculation with 50,000 or 250,000 oocysts, or 60,000 free sporocysts of the same species obtained from an Ottoman viper, Vipera xanthina xanthina. The earliest developmental stages were seen on day 8 post-inoculation (PI) and consisted of two types of meronts and gamonts (undifferentiated sexual stages). Gamonts, microgametocytes, macrogametes, and unsporulated oocysts were found on days 10 and 12 PI. Fully sporulated, thin-walled oocysts containing eight sporozoites surrounded by a thin sporocyst membrane were first seen 12 days PI. Monozoic cysts (caryocysts) were first seen 12 days PI and appeared fully viable throughout the duration of the study, 70 days PI. Four mice injected intra-peritoneally with 150,000 free sporozoites and killed 12 days PI contained unsporulated and sporulated oocysts in connective tissues of the cheek, tongue, and nose, suggesting that sporozoites may be carried to the site of infection via the lymphatic/circulatory system. Four cotton rats, Sigmodon hispidus, inoculated orally with 250,000 oocysts all had unsporulated and sporulated oocysts of C. simplex in connective tissue of the cheek, tongue, and nose when killed on day 12 PI, indicating extraintestinal development in the secondary host is not species specific. This is the first report of a heteroxenous coccidium with both asexual and sexual development in the primary (predator) and secondary (prey) hosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb02985.x
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  • 2
    Electronic Resource
    Electronic Resource
    In Vitro Excystation of Caryospora simplex (Apicomplexa: Eimeriorina) (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In vitro excystation of sporozoites of the heteroxenous coccidian Caryospora simplex Léger, 1904 (Apicomplexa: Eimeriorina) is described. Sporocysts freed mechanically from oocysts released a maximum of 51% of their sporozoites within 45 min at 25°C and a maximum of 74% within 20 min at 37°C when incubated in a 0.25% (w/v) trypsin–0.75% (w/v) sodium taurocholate (bile salt) excystation solution. At emergence from sporocysts, sporozoites were weakly motile then became highly active after about 2 min in excystation solution. Sporozoites within sporocysts exposed to bile salt only became highly motile within 25 min at 25°C and within 15 min at 37°C but did not excyst. When exposed only to trypsin at the above temperatures, the Stieda body dissolved; the substieda body remained intact, and the sporozoites exhibited only limited motility within sporocysts; only a few excysted. Intact, sporulated oocysts incubated at 25° or 37°C in 0.02 M cysteine-HC1 and a 50% CO2 atmosphere for 18 h had no morphologic changes in the oocyst wall. Further incubation of these intact oocysts in excystation solution for 30 min at 37°C caused neither motility of sporozoites within sporocysts nor excystation. Grinding oocysts for 30 sec in a motor-driven, teflon-coated tissue grinder caused motility of some sporozoites within sporocysts but did not result in excystation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb02964.x
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  • 3
    Electronic Resource
    Electronic Resource
    Development of Caryospora simplex (Apicomplexa: Eimeriidae) from Sporozoites to Oocysts in Human Embryonic Lung Cell Culture (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Leighton tubes containing monolayers of human embryonic lung cells were inoculated with 70,000 or 30,000 sporozoites of the viperid coccidium Caryospora simplex and examined at 1, 2, 4, 6, 8, 10, 12, 14, 16, and 18 days post-inoculation (PI). By day 1 PI, sporozoites had penetrated cells and were within parasitophorous vacuoles. Most sporozoites became spherical and then underwent karyokinesis several times between days 2 and 6 PI. Mature Type I meronts were found on days 6–16 PI and contained 8 to 22 short, stout merozoites. Mature Type II meronts were present on days 10–18 PI and contained 8 to 22 long, slender merozoites. Developing gamonts (undifferentiated sexual stages) were observed on days 14 and 16 PI. Mature micro- and macrogametes and thin-walled unsporulated oocysts were present on days 16 and 18 PI. Attempts to sporulate oocysts in tissue culture medium or in a 2.5% (w/v) aqueous solution of K2Cr2O7 at 25/°C and 37°C were unsuccessful; only a few oocysts developed to the contracted sporont stage. Four Swiss-Webster mice injected intraperitoneally with merozoites obtained from Leighton tubes on day 10 PI did not acquire infections. This is the second coccidium reported to complete its entire development, from sporozoite to oocyst, in cell culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb02986.x
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  • 4
    Electronic Resource
    Electronic Resource
    Complete Development of Isospora suis of Swine in Chicken Embryos (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Development of the swine coccidium, Isospora suis, in embryonated chicken eggs is described. The allantoic cavities of eight-to-ten-day-old white Leghorn embryos were inoculated with either 100,000 or 200,000 sporozoites. Developmental stages morphologically similar to those found in the intestines of piglets were present in the endodermal layer of the chorioallantoic membrane (CAM), beginning three days post inoculation (PI). No stages were found in the mesodermal or ectodermal layers of the CAM and none were observed in heart, lung, liver, or spleen. Type I meronts and merozoites were found on days 3 through 10 PI. Type II meronts and merozoites were found days 4 through 10 PI. Mature microgamonts, macrogamonts, and oocysts were found on days 7 through 10 PI. Oocysts appeared to be retained in the endodermal cells and in ovo sporulation did not occur. Attempts to sporulate CAM-derived oocysts were not successful. Isospora suis was not pathogenic for embryos under the conditions of this study. This study represents the first fully documented report of complete development of a mammalian coccidium in chicken embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb04306.x
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  • 5
    Electronic Resource
    Electronic Resource
    Cryptobia Sp. In the Snail Triadopsis Multilineata (Say): Fine Structure of Attached Flagellates and Their Mode of Attachment to the Spermatheca (1980)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 27 (1980), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A Cryptobia sp. that appears to differ structurally from Cryptobia helicis Leidy occurred as 2 distinct populations within the spermatheca of the land snail Triadopsis multilineata (Say). Attached flagellates were a resident population within the spermatheca while pleomorphic, free-swimming forms were a transient population moving freely within the reproductive tract. Slender, free-swimming flagellates were apparently the ones transmitted by a venereal route. It was observed by electron microscopy that attachment was achieved through an extensive morphologic transformation of the first 5 to 10 μm of the anterior flagellum which resulted in the formation of numerous pseudopodium-like structures (“flagellapodia”) capable of invading the microvillus border of the host tissue. Flagellapodia were delimited by a single unit membrane, contained granular cytoplasm, and were long, branched, finger-like structures which interdigitated extensively with host cell microvilli. Fine structure of the Cryptobia sp. is distinctly different from that reported for C. helicis. the differences include structure and position of the preoral ridge, the cytostome-cytopharynx, the recurrent flagellum, and the presence of the flagellapodia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1980.tb04257.x
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  • 6
    Electronic Resource
    Electronic Resource
    Eimeria forresteri n.sp. (Apicomplexa: Eimeriidae) from Ramphastos toco and a redescription of Isospora graculai from Gracula religiosa (1984)
    Springer
    Systematic parasitology 6 (1984), S. 237-240 
    Details
    ISSN: 1573-5192
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Eimeria forresteri n.sp. is described from Ramphastos toco (Piciformes: Ramphastidae) and Isospora graculai Bhatia, Chauhan, Arora & Agrawal, 1973 is redescribed from Gracula religiosa (Passeriformes: Sturnidae). E. forresteri oocysts are ellipsoid, 24.5 by 17.8 μm (21.0 to 27.0 by 16.0 to 19.5), and have a trilayered wall about 1.5 μm thick. One to three polar granules are present; micropyle and oocyst residuum are absent. Sporocysts are ellipsoid, 14.8 by 7.3 μm (12.0 to 17.0 by 6.0 to 8.0), with a smooth, thin wall. Stieda and substieda bodies and sporocyst residuum are present. Sporozoites measure 9.2 by 3.4 μm (6.0 to 12.0 by 3.0 to 4.5), with anterior and posterior refractile bodies. I. graculai oocysts are subspherical, 24.6 by 22.4 μm (20.0 to 27.8 by 20.0 to 24.0), and have a single-layered wall about 1.5 μm thick. One or two polar granules are present; micropyle is absent. Oocyst residuum is sometimes present, consisting only of a few indistinct granules. Sporocysts are ovoid, 17.7 by 11.6 μm (15.0 to 20.0 by 10.0 to 13.0), with a thin, smooth wall. Stieda and substieda bodies and sporocyst residuum are present. Sporozoites measure 14.5 by 3.5 μm (12.0 to 16.5 by 3.0 to 3.8), with anterior and posterior refractile bodies. ac]19831122
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00009233
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  • 7
    Electronic Resource
    Electronic Resource
    Excystation ofIsospora suis Biester, 1934 of swine (1983)
    Springer
    Parasitology research 69 (1983), S. 27-34 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The in vitro excystation of sporozoites ofIsospora suis Biester 1934 is described. Sporocysts ofI. suis lack a Stieda body. Upon incubation in 0.75% sodium taurocholate or in 0.25% trypsin+0.75% sodium taurocholate excystation solutions, sporozoites were released by separation of the sporocyst wall into four plates. Occasionally, the sporocyst wall did not separate completely but opened partially and released the sporozoite. At the time of excystation, sporozoites were short and broad but became elongated after 5 to 10 min in the excystation fluids. Elongate sporozoites measuring 11.7×3.8 μm, had a pointed anterior end and a nucleus located in the posterior half of the cell. Living sporozoites exhibited gliding movements, side-to-side flexion, and probed with their anterior ends. Incubation in 5.25% sodium hypochlorite removed the oocyst walls from most oocysts. Sporozoites did not excyst from sporocysts that were released during treatment with sodium hypochlorite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00934007
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