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  • 1980-1984  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Mitochondrial transformation of mammalian cells (1982)
    [s.l.] : Nature Publishing Group
    Nature 295 (1982), S. 605-607 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The antibiotics chloramphenicol and efrapeptin inhibit mitochondrial protein synthesis and ATPase, respectively, thereby killing sensitive (wild-type) mammalian cells. Various mutant cell lines have been isolated2'4 which are resistant to these drugs; we used these mutants to investigate the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/295605a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Techniques for purifying L-cell karyoplasts with minimal amounts of cytoplasm (1980)
    Springer
    Somatic cell and molecular genetics 6 (1980), S. 455-464 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mouse L-929 cells centrifuged in the presence of cytochalasin B (CB) separate into cytoplasmic (cytoplasts) and nuclear (karyoplasts) fractions, but both of these fractions contain some whole-cell contaminants. We describe here two techniques for eliminating most of these whole cells from the karyoplast population. The first involves incubating cells in the presence of tantalum (Ta) particles, followed by enucleation and separation, on a Ficoll gradient. The small karyoplasts separate from the whole cells which contain a greater number of Ta particles. The second procedure uses a cell sorter that allows purification of karyoplasts from whole cells on the basis of light scatter. Both methods remove the whole-cell contaminants and the larger, and possibly viable, karyoplasts from the smaller karyoplast population. These purified karyoplasts are unable to regenerate but may be rescued by fusion with cytoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01539149
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Detrimental effect of mitochondria on hybrid cell survival (1982)
    Springer
    Somatic cell and molecular genetics 8 (1982), S. 15-21 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A genetic technique has been developed for isolating nuclear hybrids which are products of fusion between karyoplasts and whole cells. Nuclear hybrids have a greatly reduced mitochondrial component obtained from the nuclear donor and exhibit differences when compared to the corresponding whole-cell hybrids, such as better long-term survival and accelerated chromosome segregation. These results suggest that divergent mitochondria in a cell-fusion product may have adverse effects on survival.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538647
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  • 4
    Electronic Resource
    Electronic Resource
    Suppression of tumorigenicity in cybrids (1981)
    New York, NY : Wiley-Blackwell
    Journal of Supramolecular Structure and Cellular Biochemistry 16 (1981), S. 75-82 
    Details
    ISSN: 0275-3723
    Keywords: cytoplasmic hybrids ; suppression of tumorigenicity ; Chemistry ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We investigated cytoplasmic control of tumorigenicity in cybrids. Cytoplasts derived from nontumorigenic cells were fused to the highly tumorigenic 984 Cl 10-15 cell line derived from a murine teratoma. The resultant cybrids did not retain the tumorigenicity of the original cell line. In addition, the majority demonstrated the ability to differentiate into skeletal muscle. The results of these experiments indicate a heritable suppression of the tumorigenic phenotype by nontumorigenic cytoplasm. These findings are in contrast to our previous experiments in which we used a different experimental system and demonstrated a nuclear control of tumorigenicity in cybrids.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jsscb.1981.380160107
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