ISSN:
1573-5044
Keywords:
Dalbergia
;
clonal multiplication
;
nodal explants
;
tissue culture
;
elite trees
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Multiple shoots were obtained from nodal explants of 30-year-old trees of Dalbergia sissoo Roxb. on a defined medium, MS (Murashige & Skoog medium) supplemented with auxin-cytokinin combinations. IAA (Indole accetic acid) alone promoted 15% rooted shoot buds. A combination of IAA+Kn (Kinetin) gave 100% rooted shoot buds. A combination of NAA (napthaleneacetic acid) + Kn and NAA+BAP (6-benzylaminopurine) also gave high percentages of rooted shoot buds. Ascorbic acid in the medium prevented the death of callus and plantlets, which followed darkening of the medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033548
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