ISSN:
1432-203X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cotyledonary nodes of corylus avellana L. excised from young seedlings cultured in K(h) medium for 5 weeks were used to induce embryoid formation. Embryogenesis was achieved in 60% of the explants over two 20-day culture steps in the presence of IBA (5 μM) plus BAP (0.5 μM) and BAP (5 μM) plus IBA (0.5 μM) respectively. Subsequent proliferation was successfully maintained during 5 subcultures on K(h) basal medium and in an unlimited number of subcultures in the presence of BAP (0.5 μM). Plant regeneration (50–55%) was easily achieved by planting into a basal K(h) medium after 10–20 days.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00269146
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