ISSN:
1573-2592
Keywords:
Serum antibodies
;
ELISA
;
A. actinomycetemcomitans
;
leukotoxin
;
periodontal disease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Human serum antibody responses to antigens from a suspected oral pathogen,Actinobacillus actinomycetemcomitans (Aa), were studied. IgG and IgM isotype anti-bodies to four antigen preparations, sonicate antigen (SA), leukotoxin (LT), group carbohydrate (LG), and lipopolysaccharide (LPS), were determined using an ELISA. An ELISA inhibition technique was developed to show that human serum antibodies reacting with the LT, LG, or LPS materials were binding to different antigenic moieties in each preparation. Cross-sectional studies of serum IgG antibodies showed that patients with localized juvenile periodontitis (LJP) had a greater frequency of occurrence and a higher level of antibodies to the SA (82%), LT (70%), and LG (62%) antigens compared to all other diseased (11–46%) or normal (4–13%) groups. Serum IgM antibodies to LPS were increased in LJP, generalized juvenile periodontitis, and adult periodontitis patients compared to all other groups. Therefore, while both IgG and IgM antibodies were found against various Aa antigens, the detection of IgG antibodies was most clearly associated with the specific disease classification of LJP. Blocking studies suggested that the human serum responses were specific for the Aa antigens and that the LT, LG, and LPS comprise major antigenic determinants on the organisms to which human serum antibody reacts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00915793
Permalink
