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  • 1
    Electronic Resource
    Electronic Resource
    The potential of pre-columns to improve detection properties in high-performance liquid chromatography (1982)
    Springer
    Chromatographia 15 (1982), S. 453-458 
    Details
    ISSN: 1612-1112
    Keywords: Pre-columns ; High-performance liquid chromatography ; Plasma and serum ; Soil extracts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The potential of pre-columns to increase the selectivity and sensitivity in high-performance liquid chromatography is demonstrated by some case studies. An on-line pre-column of 5 cm was used for the determination of fluvoxamine and clovoxamine in plasma. In this example, the principle of using different packing materials in precolumn and analytical column is shown to be promising for certain applications of pre-columns for clean-up and pre-concentration. An on-line pre-column containing a 1–2 mm layer of stationary phase was successfully applied for the determination of secoverine in plasma and serum. Direct injection of 200–1000 μl of the sample is possible. This study illustrates the high selectivity obtained by the combined use of a pre-column and an extraction detector. Sep-Pak C18 cartridges were used both for clean-up and pre-concentration of soil extracts in order to isolate radio-labelled pesticides. In this way, the pretreatment of such complex samples is very rapid and simple.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261608
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Optimization of a peroxyoxalate chemiluminescence detection system for the liquid chromatographic determination of fluorescent compounds (1984)
    Springer
    Chromatographia 18 (1984), S. 129-133 
    Details
    ISSN: 1612-1112
    Keywords: Chemical excitation source ; Chemiluminescence detector ; Chemical band narrowing ; Dansyl derivative ; Serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A peroxyoxalate chemiluminescence detection system for liquid chromatography is described. The excitation of fluorophores is generated by the reaction of bis-(2,4,6-trichlorophenyl)oxalate or bis(2,4-dinitrophenyl)oxalate and hydrogen peroxide, which are added to the column effluent. The influence of the solvents and the concentrations of the reagents have been investigated. The influence of the flow cell volume on sensitivity and on band broadening have also been studied and a “chemical band narrowing effect” has been observed. Different types of apparatus have been compared for detection of the emitted light. The system has been used for the detection of the dansyl derivative of a drug with a secondary amine functional group in serum samples. The detection limits are in the 1–10pg range.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258768
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of some parameters on the liquid chromatographic separation of biogenic amines and their metabolites (1981)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 444-447 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; Reversed-phase ion-pair system ; Biogenic amines and metabolites ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The liquid chromatographic separation of some biogenic amines and their metabolites with a reversed-phase ion-pair system has been optimized. The effects of the nature of the stationary phase, the column temperature, the pH, and the ion-pair reagent concentration of the mobile phase have been investigated. The most striking result is the influence of the column temperature on selectivity.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240040904
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    Paper (German National Licenses)
    Fulltext
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