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  • 1
    Electronic Resource
    Electronic Resource
    Distribution of endothelial and basement membrane markers in angiogenic tumors of the nervous system (1983)
    Springer
    Acta neuropathologica 62 (1983), S. 67-72 
    Details
    ISSN: 1432-0533
    Keywords: Factor-VIII-related antigen ; Lectin ; Laminin ; Angiogenic tumor ; Endothelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution of two endothelial cell markers Factor-VIII-related antigen and Ulex europaeus agglutinin was examined by immunoperoxidase and immunofluorescence techniques in paraffin-embedded specimens representing the three main types of angiogenic neoplasms of the nervous system, hemangioblastoma, hemangioendothelioma and hemangiopericytoma. In addition, the distribution of the basement membrane (BM) marker, laminin, was studied in the same tumors. It was found that Ulex europaeus agglutinin was a more sensitive marker of neoplastic endothelial cells than Factor-VIII-related antigen. Both markers only stained endothelial cells, while the tumor cells of hemangiopericytomas and the stromal cells of hemangioblastomas remained unstained. These findings do not support the view that the stromal cells of hemangioblastomas are derived from endothelial cells. With antiserum to laminin a typical staining pattern could be noticed in each tumor, showing the architectural relationships of the cells very clearly. In all three tumor types laminin was only found in the BM of the vessels, not in the interstices of the neoplastic cells outside vessel lumina. Therefore, the reticulin network previously found between the individual cells of hemangiopericytomas does not correspond to BM. It is concluded that both Ulex europaeus agglutinin and laminin antisera could be valuable new aids for the diagnosis of the three tumor types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684922
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of a major connective tissue protein, fibronectin, in normal and neoplastic human nervous tissue (1980)
    Springer
    Acta neuropathologica 51 (1980), S. 47-51 
    Details
    ISSN: 1432-0533
    Keywords: Fibronectin ; Glial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fibronectin is a cell surface-associated glycoprotein of various adherent cells in culture, and it has been found to be a major component of connective tissue matrix in human tissues. Reports on expression of fibronectin by cultured human astroglial cells prompted us to study its distribution in vivo. In human brain tissue fibronectin was located, as studied by immunofluorescence staining, to capillary vessel walls in a pattern corresponding both to the vessel lumen and the basement membrane of vessel wall. Glial or neuronal cells did not express fibronectin in the adult human brain. In human brain tumors of the glioma group occasional faint intercellular fluorescence was observed, probably due to leakage of fibronectin from the blood stream. A strong staining associated with the endothelial glomerulus-like proliferations was also observed in malignant gliomas. In peripheral nerves fibronectin fluorescence corresponding to the basement membranes of perineurial and Schwann cells was seen. An interesting feature was the intense staining at the nodes of Ranvier. In a well differentiated schwannoma of peripheral nerve an intercellular lamellar staining pattern was observed, corresponding to the basement membranes of the neoplastic cells. These results indicate that in the human nervous tissue fibronectin occurs only at sites of contact between the neuro-ectoderm and mesenchyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688849
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Glial uptake of monoamines in primary cultures of rat median raphe nucleus and cerebellum (1981)
    Springer
    Histochemistry and cell biology 73 (1981), S. 239-250 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Glial uptake of serotonin and dopamine was studied in primary cultures of the median raphe nucleus and cerebellum by using consecutive demonstration of monoamine fluorescence and glial fibrillary acidic protein immunofluorescence. Most of the glial cells taking up monoamines were glial fibrillary acidic protein positive. Astrocytes with a strong immunoreactivity exhibited monoamine fluorescence only occasionally, although such cells did take up L-dopa readily. Glial fibrillary acidic protein negative cells — morphologically identified as astrocytes — were seen to exhibit monoamine fluorescence after exposure. Glial uptake of serotonin at a concentration of 10−4 M was detected in cerebellar cultures but not in cultures from the median raphe nucleus. When the concentration was 10−3 M uptake of serotonin took place in both the areas but was weaker in cultures from the median raphe nucleus. At concentrations greater than 10−5 M glial uptake of dopamine was detected in cultures from both the regions studied. No region dependent differences in glial uptake of dopamine was demonstrated, however. Based on these observations astrocytes and astrocyte-like glial cells take up dopamine and serotonin. Also glial cells with a remarkably high content of the glial fibrillary acidic protein are more resistant to monoamine uptake than cells exhibiting less intense or no glial fibrillary acidic protein immunofluorescence. The existence of regional differences in uptake of serotonin between the median raphe nucleus and cerebellum suggests that glial uptake of monoamines is not an entirely passive mechanism but may be actively controlled by glial cells in a region dependent manner.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493024
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    Paper (German National Licenses)
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