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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 116 (1983), S. 75-77 
    ISSN: 1615-6102
    Keywords: Ca2+ ; Cell model ; Cytoplasmic streaming ; Nitella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytoplasmic streaming in permeabilizedNitella cells was found to be controlled by Ca2+ of physiological concentration. The streaming driven by Mg · ATP was scarcely affected by 10−7 M Ca2+, but was inhibited significantly by 5 × 10−7 M Ca2+ and completely by 10−6 M Ca2+. The inhibition by Ca2+ was completely reversible even at 10−5 M.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 109 (1981), S. 103-111 
    ISSN: 1615-6102
    Keywords: Calcium ions ; Chara ; Cytoplasmic streaming ; Internal perfusion ; Tonoplast-free cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of the intracellular concentration of Ca2+ on the cytoplasmic streaming of tonoplast-free cells ofChara australis was studied by intracellular perfusion. The perfusion media contained 1 mM Mg · ATP. Both cell ends were cut and left open. Media of different Ca2+ concentrations were perfused through the cell and the rate of the cytoplasmic streaming just after perfusion was measured. The critical concentration of Ca2+ for inhibiting the streaming was 5 × 10−4M, which was substantially higher than that found earlier byWilliamson (1975) andHayama et al. (1979). Recovery from the inhibition occurred, though not completely, by removing Ca2+. In tonoplast-free cells the Ca2+ sensitivity differed according to the culture conditions. Cells cultured indoors exhibited a higher sensitivity than those cultured outdoors. Theformer cells contained granule-rich endoplasm aggregates after loss of the tonoplast, while the latter cells did no such aggregates. The aggregates were fixed to the cortical gel with a high dosage of Ca2+ and freed by removing it.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6102
    Keywords: Actin filaments ; Chara ; Cytoplasmic streaming ; Internal osmotic pressure ; Nitella ; Tonoplast-free cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of osmolarity of the vacuolar sap ofChara australis on cytoplasmic streaming was analyzed using the vacuolar perfusion technique. The osmolarity was varied between 0.3 M, which is normal and 1.2 M. The streaming rate decreased markedly with an increase in sap osmolarity, while the motive force increased significantly. This may be explained in terms of an increase in the sliding resistance at the sol-gel interface where active shearing occurs. Increase in the resistance is assumed to be caused by osmotic dehydration of the cytoplasm. This assumption was verified by the fact that in tonoplast-free cells, no significant inhibition of the streaming was observed by heightening the osmolarity of the cytoplasm with sorbitol. Heightening it with K+ salts inhibited the streaming to a greater extent than with sorbitol. The inhibition differed according to the anion species. Potassium methanesulfonate at 0.3 M and KCl at 0.6 M stopped the streaming almost completely, while 0.59 M K2SO4 was less inhibitory. Actin filaments were observed even in the presence of 0.6 M KCl.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1615-6102
    Keywords: Action potential ; Ca2+ ; Chara ; Cytoplasmic streaming ; EGTA ; Nitella ; Permeabilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When K+ of high concentration (50 mM) was applied toNitella cells, the cytoplasmic streaming stopped instantly as in the case of electrical stimulation. Recovery of the streaming after chemical stimulation was much slower than after electrical stimulation. When the endoplasm content was modified by centrifugation, streaming recovery was accelerated in the centrifugal cell fragments rich in endoplasm and deccelerated in those poor in it. The recovery was also accelerated either by permeabilizing the plasmalemma in the presence of EGTA in the external solution or by removing the tonoplast by vacuolar perfusion with the EGTA-containing medium. We concluded that the streaming was recovered due to decrease of the cytoplasmic Ca2+ concentration, which seems to be accelerated by sequestering of Ca2+ by endoplasmic components. The slow recovery of the streaming after KCl-stimulated cessation is assumed to be caused by continuous influx of Ca2 + during the prolonged membrane depolarization.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 226 (1982), S. 63-73 
    ISSN: 1432-0878
    Keywords: Hygroreceptor ; Thermoreceptor ; Coelocapitular sensillum ; Honey bee ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antennal hygroreceptors of the honey bee, Apis mellifera L., have been investigated electrophysiologically and the sensillum containing these receptors with SEM. Moist and dry hygroreceptors have been identified along with a thermal receptor in a specialized coeloconic sensillum. This sensillum comprises a cuticular, shallow depression (diameter; 4 μ) having a central opening (1.4–1.5 μm) and a mushroom-shaped protrusion (1.4–1.5 μm) from the opening. The head of the protrusion is irregular in shape and is not perforated. This sensillum has been thus far referred to as a “sensillum campaniformium” (Dietz and Humphreys 1971), henceforth, it is referred to as a coelocapitular sensillum. The responses of both moist and dry hygroreceptors are of a phasic-tonic manner. Both receptors are antagonistic with respect to their responses to humidity; one responds with an increase in impulse frequency to rising humidity, the other to falling humidity. The humidity-response relationship is independent of stimulus flux.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 226 (1982), S. 309-318 
    ISSN: 1432-0878
    Keywords: Hygroreceptive sensillum ; Cockroach ; Antenna ; External structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The antennal hygro- and thermoreceptive sensillum (sensillum capitulum) of the cockraoch, Periplaneta americana, has been identified electrophysiologically and examined by scanning electron microscopy. It appears basiconic and has a characteristic cuticular apparatus; a mushroom-shaped inner stem and a non-perforated outer wall. The head of the inner stem, which is a cap of about 0.5 μm diameter, protrudes from the distal margin of the outer wall. The inner stem is not perforated, though in some cases a tiny hollow is observed in the apex of the cap. This kind of cuticular apparatus is not found in other sensilla and may be modality-specific to hygro- and/or thermoreception. The sensillum capitulum occurs singly on the distal part of each alternating segment of the flagellum and on each segment of some distal meristal segments.
    Type of Medium: Electronic Resource
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