ISSN:
1573-4943
Keywords:
peptide synthesis
;
Fmoc protecting group
;
Gramicidin B
;
channel properties
;
physical characterization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The solid phase synthesis of [[1-13C]Phe11]Gramicidin B was successfully achieved using the 9-Fluorenylmethyloxycarbonyl protecting group. There was at least a 30% drop in yield during the adition of the three valines in positions 8, 7 and 6, which is likely due to steric hindrance caused by the steric constraints of the valine side chains. Still, the overall yield of the peptide was comparable with that obtained using thetert-butyloxycarbonyl group for protection. The synthetic Gramicidin B was completely characterized by high-pressure liquid chromatography, circular dichroism, and13C nuclear magnetic resonance spectra. Also reported are the single-channel conductance properties, which compare favorably with those reported earlier and demonstrate the interesting multiplicity of conductance states to be distinquishable from that of Gramicidin A. Thus, these several useful characterizations have been carried out on the same preparation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01024999
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