ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Crossed affinoimmunoelectrophoresis (CAIE) with free lectin in the first dimension was used to investigate sugar heterogeneity of inter-α-trypsin-inhibitor (ITI) in normal human serum and after purification. Such a heterogeneity was assessed with Lens culinaris agglutinin and Concanavalin-A, but not with wheat germ agglutinin. The use of a mixture of purified ITI and ITI-free serum demonstrated that a distortion of the immunoprecipitation patterns for ITI in normal serum was observed with Con-A and WGA and was due to presence of other glycoproteins. Thus, prior protein purification is sometimes required before obtaining a true pattern of glycoprotein microheterogeneity by lectin CAIE. Furthermore, loading a sugar specific for the lectin in the second dimension gel was shown to be necessary in order to observe all heterogeneous forms of the glycoprotein tested.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150010315
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