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  • 1980-1984  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Determination of urea in 10-μl blood serum samples with a urease reactor / ion-selective electrode cell (1983)
    Springer
    Microchimica acta 81 (1983), S. 135-147 
    Details
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Ein Meßsystem, bestehend aus einem miniaturisierten Enzymreaktor und einer neuartigen Ammonium-Minielektrode, wurde für Harnstoffbestimmungen in 10-μl-Proben entwickelt. Im Hinblick auf eine hohe Reproduzierbarkeit der Meßwerte wurde ein nichtkontinuierliches Verfahren erarbeitet. Die Parameter des Meßverfahrens wurden sorgfältig optimiert. Die Anwendbarkeit des Harnstoff-Analysators wurde an 122 Blutserumproben von Patienten überprüft. Die Ergebnisse der enzymatisch-potentiometrischen Bestimmungsmethode korrelierten ausgezeichnet mit jenen der Diacetylmonoxim-Referenzmethode. Die relative Streuung um die entsprechenden Regressionsgeraden (Steigung 1.0) betrug ±6% für einen Harnstoff-Konzentrationsbereich von 1–45 mM.
    Notes: Summary A system consisting of a miniaturized enzyme reactor and a novel ammonium-selective minielectrode was designed for urea determinations in 10-μl samples. A discontinuous technique was devised in view of a high measurement reproducibility. The parameters of the measuring procedure were carefully optimized. The applicability of the urea analyzer was tested using 122 blood serum samples from patients. The results of the enzymatic/potentiometric method agreed favorably with the reference values determined by the diacetyl monoxime method. The relative residual standard deviation found for such linear correlations (slope 1.0) was ±6% over the 1 to 45 mM urea range.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01202688
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Substrate-conversion efficiency, kinetic parameters, and life time of an urease reactor (1983)
    Springer
    Microchimica acta 81 (1983), S. 171-181 
    Details
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Ergebnisse einer theoretischen Abhandlung über Enzymreaktoren und Enzymelektroden werden zusammengefaßt und die Anwendungsbereiche des Modells diskutiert. Das Leistungsverhalten eines miniaturisierten Urease-Reaktors (in Kombination mit einer ionenselektiven Elektroden-Meßzelle) wurde auf der Grundlage dieser Theorie analysiert. Der frisch hergestellte Reaktor bewirkte in Substratlösungen einen Reaktionsumsatz von ∼100%. Das Altern des Reaktors wurde quantitativ untersucht, indem die Meßwerte mit berechneten Kurven verglichen und die entsprechenden kinetischen Parameter abgeleitet wurden. Als limitierend für die Lebensdauer des Reaktors erwies sich die nach einem exponentiellen Zerfallsgesetz erfolgende Abnahme der Enzymaktivität.
    Notes: Summary The key results of a theoretical model of immobilized-enzyme reactors and enzyme electrodes are summarized, and the applicability of the theory is discussed. The model was used to analyze the performance of a miniaturized urease reactor (coupled to an ionselective electrode cell). For the freshly prepared reactor a substrate-conversion efficiency of ∼100% was established. Aging of the reactor was quantitatively studied by fitting theoretical curves to experimental response data and evaluation of the kinetic parameters. The life time turned out to be limited by an exponential decay of enzyme activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01497608
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Kinematic data acquisition system for two- or three-dimensional motion analysis (1981)
    Springer
    Medical & biological engineering & computing 19 (1981), S. 287-290 
    Details
    ISSN: 1741-0444
    Keywords: 3-D motion analysis ; Film drive unit ; L.E.D. markers ; Still camera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This paper presents a novel data acquisition svstem for motion analysis in two or three dimensions. Emphasis is placed on a relatively simple method of data collecting requiring minimal equipment. The system consists of an ordinary 35 mm camera (one camera for two dimensions or two cameras for analysis in three dimensions), infrared light emitting diodes (l.e.d) used as markers, and an electronic control circuit. The camera records successive positions of pulsed infrared l.e.d.s. which are attached over anatomical landmarks, to produce sequences of dots on the film. The resulting trajectories of the flashing diodes are digitised to generate numerical values which serve as input data to the computer carrying out the actual motion analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02442546
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    Paper (German National Licenses)
    Fulltext
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