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  • 1975-1979  (5)
  • 1970-1974  (1)
  • 1965-1969  (1)
  • Cell & Developmental Biology  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Corpus luteum growth and plasma progesterone levels in unilaterally ovariectomized pregnant rats (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 311-316 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Unilateral ovariectomy (ULO) was carried out on rats at Day 8 of gestation: term is Day 23. The effect on peripheral plasma progesterone levels at Day 16 and on growth and cellular changes in the remaining corpora lutea (CL) at Day 17 was determined by comparison with results from control and sham-operated rats. ULO increased the volume of remaining CL by 18% at Day 17. At Day 16 this effect was only apparent in rats with three or fewer CL remaining. CL hypertrophy was due to an increase in the volume of individual luteal cells and not to cell hyperplasia. Luteal cell nuclear volume also increased as did the number of endothelial cells per corpus luteum. The proportion of the corpus luteum occupied by vascular space did not alter.ULO had little effect on mean plasma progesterone levels at Day 16 (92 ± 7 ng/mltreated; 86 ± 11 ng/ml controls). In rats with three or fewer CL remaining, however, progeserone levels were low (51 ± 7 ng/ml).It was concluded that ULO can enhance the growth of corpora lutea and perhaps increase their rate of progesterone production.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950206
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  • 2
    Electronic Resource
    Electronic Resource
    Cytological events associated with in vitro aged and fertilized rabbit eggs (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 357-374 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The morphogenetic events associated with rabbit eggs aged in vitro for 12 to 50 hours prior to mixing with sperm have been examined by light and electron microscopy. After 12 hours in culture, morphological alterations of the meiotic spindle and the cortex of unfertilized eggs were evident. By 24 to 50 hours in culture, unfertilized eggs contained subnuclei, structures which formed when individual and/or groups of meiotic chromosomes dispersed and became invested by a double-laminated structure reminiscent of a nuclear envelope.Although most eggs obtained 11.5 to 12 hours after induced ovulation and in vitro fertilized displayed morphogenetic patterns similar to those described for in vivo fertilized ova, some (10%) contained three pronuclei. Many eggs obtained 13 to 15 hours after induced ovulation and subsequently mixed with sperm in vitro appeared to undergo processes of fertilization typical of in vivo fertilized eggs, however, approximately 30% contained subnuclei in association with the male pronucleus. Few eggs (15%) aged 12 hours prior to in vitro fertilization displayed patterns of pronuclear development and association typical of fertilized unaged ova. Subnuclei developed in many of the fertilized ova. Supernumerary sperm nuclei, which did not develop into male pronuclei, were observed in some zygotes. Cleavage of eggs aged 12 hours prior to fertilization was abnormal or retarded. After 24 hours in culture approximately 16% of the eggs fertilized. Seventy percent of the fertilized eggs failed to Support the development of a male or female pronucleus.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950209
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural changes of rat blastocysts induced by estrogen during delayed implantation (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 179 (1974), S. 253-271 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ultrastructural changes of rat blastocysts during delayed implantation were studied 16, 20, 24 or 30 hours after estrogen was given to induce implantation.In the inner cell mass the presence of long cytoplasmic processes penetrating deeply into the neighboring inner cell mass cells is seen at 16 hours. Most cells also show an increased number of ribosomes, polyribosomes and granular endoplasmic reticulum.The trophoblast is featured by the formation of large amounts of glycogen and many inclusion bodies. Glycogen granules appear first in some abembryonic trophoblast cells at 16 hours, and spread to the embryonic pole at 24 hours. New inclusion bodies appear sequentially: multivesicular bodies at 20 hours, multigranular bodies at 24 hours and lamellar bodies at 30 hours. The functions of these inclusion bodies remain to be studied.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091790208
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  • 4
    Electronic Resource
    Electronic Resource
    The cellular pattern of corpus luteal growth during pregnancy in the rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 823-830 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cellular pattern of corpus luteal (CL) growth was studied in rats at Days 6, 10, 12, 14, 16, 17 and 22 of pregnancy: term is Day 23. Measurements were taken of the percentage of the CL occupied by luteal cells, connective tissue and vascular space, luteal cell and nuclear volumes, and the number of luteal and endothelial cells in each of three CL in both ovaries of five rats at each stage of pregnancy. Total CL volume increased from 1.08-3.23 μl over Days 10-17. This was mainly due to an increase in luteal cell volume from 3.72 pl to 9.30 pl. Neither the number of luteal cells per CL (range 212,000-287,000) nor the percentage of the CL occupied by luteal cells (range 85-90%) had much influence on growth. Nuclear volume increased roughly in proportion to cytoplasmic volume but near term it decreased despite little change in cytoplasmic volume. The number of endothelial cells per CL increased steadily from 398,000 at Day 6 to 1,545,000 at Day 22. There was a strong negative correlation (r = -0.78) between the number of luteal cells per CL and mean luteal cell volume that was evident at all stages of pregnancy. There was a positive, but weaker correlation (r = 0.35) between number of luteal cells and CL volume. Thus, CL volume seems to be partly determined by the number of luteal cells at Day 6 but this effect is moderated by local control of luteal cell volume.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930406
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  • 5
    Electronic Resource
    Electronic Resource
    The visual cells of the chicken as revealed by phase contrast microscopyThis investigation was supported, in part, by research grant AM 06705 from the National Institutes of Health, and, in part, by a Fight for Sight Grant-in-Aid of the National Council to Combat Blindness, Inc., New York, New York. (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 118 (1966), S. 723-734 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Phase microscopic investigations of Kolmer-fixed, depigmented sections of the adult chicken retina have provided photomicrographic evidence of the existence of three different photoreceptors: single rods, single cones, and double cones. The rod extends the entire thickness of the visual cell layer and is characterized by a uniformly thick outer segment and a hyperboloid-containing inner segment which is devoid of an oil droplet. The single cone is the shortest element; it contains a red oil droplet. The double cone consists of two unequal members, a tall, slender chief cone and a broad accessory cone. The chief component contains a large yellow oil droplet, whereas the accessory cone houses a small, oval, yellowish-green droplet and a characteristically large, oval paraboloid. The rod hyperboloid and the accessory cone paraboloid contain glycogen. No colorless droplets have been observed. Owing to the close association between oil droplet color and cone type, three colored layers of oil droplets are formed within the thickness of the retina: a proximal row of red droplets (the short, single cones), an intermediate layer of yellowish-green droplets (the accessory cones), and a distal row of yellow droplets (the tall chief cones).
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001180303
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  • 6
    Electronic Resource
    Electronic Resource
    Microfilaments, the smooth endoplasmic reticulum and synaptic ribbon fields in the pinealocytes of the baboon (Papio ursinus)Supported by the Medical Research Council and the University of Pretoria. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 154 (1979), S. 151-161 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Microfilaments (MF, 5-8 nm in diameter) are a prominent feature of the pinealocyte cytoplasm of baboons (Papio ursinus) kept under controlled lighting conditions. MF occurred as a filamentous network in these cells during the light phase of the diurnal light-dark cycle, while a close structural association was noted between MF and the membranes of the smooth endoplasmic reticulum (SER). This association was especially evident during the dark period. Increased numbers of single synaptic ribbons (SR, vesicle-crowned rodlets), together with large aggregations of SR, i.e., ribbon fields (RF), were seen in the pinealocyte cytoplasm of baboons killed during the dark phase. It is suggested that the vesicles of RF may arise from those of the SER and that MF may play a role in the movement of SER-vesicles to those areas of the cytoplasm where new RF are being formed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001540203
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  • 7
    Electronic Resource
    Electronic Resource
    Antibody-mediated suppression of bone marrow colony formation in vitroSupported in part by USPHS Grant AI 10931 from the National Institute of Allergy and Infectious Diseases and a grant from Smith Kline and French Foundation. (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 94 (1978), S. 47-56 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Antisera to mouse brain reacts with hematopoietic stem cells in the mouse bone marrow. We have examined the effect of anti-mouse brain serum (AMBS) on the development of in vitro colonies from mouse bone marrow cells. The addition of 5% AMBS to the cultures markedly decreased the numbers of colonies formed to an average of 10% of the number obtained with normal rabbit serum. AMBS suppressed formation induced by colony stimulating factors (CSF) derived from three different sources; serum from endotoxin treated mice, mouse L-cell conditioned media, and human peripheral blood mononuclear cell conditioned media. The suppressive activity was quantitatively recovered in the IgG fraction of AMBS. Divalent F (ab′)AHBS, rabbit anti-human brain serum; AMBS, rabbit anti-mouse brain serum; BM, bone marrow; CFU-C, colony forming unit in vitro; CFU-S, spleen colony forming unit; CSF, colony stimulating factor; FCS, fetal calf serum; MEM, minimal essential medium; NRS, normal rabbit serum; PBS, 0.01 M sodium phosphate, 0.15 M sodium chloride, pH 7.4. fragments were as effective as the intact IgG in decreasing colony formation. Fab fragments were not suppressive. These results suggest that colony formation is induced via a dynamic interaction between CSF and the progenitor cell membrane, and that antibody directed at cell membrane antigen(s) interferes with the generation of the induction signal.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040940107
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