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  • 1975-1979  (3)
  • 1970-1974
  • 1950-1954
  • 1940-1944
  • 1930-1934
  • Amoeba proteus  (2)
  • Cellular immune reaction  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 54 (1976), S. 137-140 
    ISSN: 1432-1440
    Keywords: Chronic persistent and aggressive hepatitis ; Aggressive Emperipolesis ; Lymphocyte cytotoxicity ; Cellular immune reaction ; Piecemeal necrosis ; Chronische persistierende und aggressive Hepatitis ; Aggressive Emperipolese ; Lymphozyten-Cytotoxizität ; Zelluläre Immunreaktion ; Mottenfraßnekrose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In Biopsiepräparaten von chronischen Hepatitiden wurde die Penetration und Zerstörung von Hepatocyten durch Lymphocyten (aggressive emperipolese) beschrieben und quantitativ ermittelt. Bei den untersuchten 48 Fällen von chronisch-persistierender Hepatitis spielte die aggressive Emperipolese nur eine untergeordnete Rolle und fehlte in vielen Fällen gänzlich. Demgegenüber ist sie bei den 36 untersuchten Fällen von chronischaggressiver Hepatitis stets deutlich, mitunter sogar in erheblichem Ausmaß vorhanden. Die aggressive Emperipolese ist — neben den Mottenfraßnekrosen — ein morphologisches, im Leberstanzzylinder lichtmikroskopisch nachweisbares Substrat für den Prozeß der „self-perpetuation“ des chronisch-entzündlichen Geschehens und ein weiteres Kriterium für die Abgrenzung der beiden Haupttypen der chronischen Hepatitis.
    Notes: Summary The penetration and destruction of hepatocytes by lymphocytes (aggressive emperipolesis) has been described and examined quantitatively in biopsy specimens of chronic hepatitis. In 48 cases of chronic persistent hepatitis, the aggressive emperipolesis was rare and, obviously, not of great importance; sometimes there was no emperipolesis at all. In 36 cases of chronic aggressive hepatitis, however, emperipolesis was always evident, usually even to a very high extent. The aggressive emperipolesis — as well as the piecemeal necrosis — is a significant phenomenon which can be seen in the light microscope in liver biopsies and gives evidence to the fact that this chronic inflammatory process is a progressive one; furthermore, it is an additional criterion to differentiate the two main types of chronic hepatitis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 100 (1979), S. 33-43 
    ISSN: 1615-6102
    Keywords: Amoeba proteus ; Ca++-binding sites ; Cytochemical demonstration ; Induced pinocytosis ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Different methods were used to demonstrate the existence of Ca++-binding sites (Ca++-bs) at the plasma membrane ofAmoeba proteus. In pinocytoting animals the number (indicated by the average distanced in nm) and size (average longitudinal axiss in nm) of Ca++-bs at the cytoplasmic surface of the cell membrane were significantly increased (d=162±15;n=41 ands=93±5;n=47) in comparison to controls (d=208 ±21;n=37 ands=59±8;n=45). The ratio of P: Ca obtained by X-ray microanalysis was in the range of 1.5. The differences observed in the two experimental groups of amoebae are explained by conformational changes in the molecular structure and an increased Ca++-permeability of the plasma membrane during induced pinocytosis. Microplasmodia of the acellular slime moldPhysarum polycephalum investigated for comparison were found to have no Ca++-bs at the interior cell surface.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 197 (1979), S. 263-279 
    ISSN: 1432-0878
    Keywords: Induced pinocytosis ; Dynamics ; Motive force generation ; Light and electron microscopy ; Amoeba proteus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mechanism of induced pinocytosis was investigated in Amoeba proteus by light and electron microscopy. The application of nine different inducing substances revealed that pinocytotic channel formation, elongation, vesiculation, shortening and disappearance are the result of the successive or simultaneous action of both traction and pressure forces, which are produced by the contractile activity of a plasma membrane-associated layer of filaments ranging from a few hundred nm to several μ in thickness. The initial phase of channel formation is caused by traction forces according to the membrane flow concept, whereas channel elongation and vesiculation mainly result from pressure forces in conjunction with the extrusion of small hyaline pseudopodia. Shortening and disappearance of the pinocytotic channels are brought about by local contractions of the cortical filament layer in the basal region of the hyaline pseudopodia. Experiments using latex beads as marker particles together with inducing substances show that a rapid membrane turnover during pinocytosis can be excluded, and that the plasma membrane slides as an entire structure over the underlying cytoplasm.
    Type of Medium: Electronic Resource
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