ISSN:
0021-9541
Schlagwort(e):
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Medizin
Notizen:
Suspensions of rat kidney cells obtained by disaggregation of the kidney with 0.25% trypsin were separated by electrophoresis. Previously, we found a correlation between cells with histochemically demonstrable alkaline phosphatase (HDAP) and cells with brush borders which established that HDAP is a useful marker for rat proximal tubule cells (Kreisberg et al., '77). The starting suspension of cells for electrophoresis consisted of 38.4 ± 5.7% nucleated cells with HDAP, 39.8 ± 5.7% nucleated cells without HDAP, and 20.8 ± 9.2% red blood cells. After electrophoresis, the purest fraction contained 85.8 ± 3.5% nucleated cells with HDAP, 8.4 ± 2.2% nucleated cells lacking HDAP, and 5.8 ± 2.8% red blood cells; 91.9 ± 2.4% of the nucleated cells in the purest fractions had HDAP.
Zusätzliches Material:
1 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jcp.1040930121
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