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  • 1975-1979  (2)
  • 1970-1974  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Enzyme activities of plasma and selected tissues in rainbow trout Salmo gairdneri Richardson (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 7 (1975), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: This paper describes the determination in rainbow trout of the normal levels in the plasma of eight enzymes known to be significant in animal pathology. Some relationships between plasma enzyme levels and the enzyme activities in selected tissues are discussed. For LDH which is ubiquitously distributed, we chose, by way of saturation tests, the optimum concentrations of pyruvate and NADH in the assay medium. The LDH ‘isoenzyme ratio’ was determined for heart tissue, liver, and white muscle. When blood was withdrawn from caudal vessels, we observed a net increase of plasma enzyme activities, mainly of CPK and LDH, which was demonstrated to originate from surrounding muscle. Thus cardiac sampling was the only suitable way of obtaining blood for this kind of study. Slightly haemolyzed blood appeared suitable for enzyme determinations except for Alk Pase which is about 50 times more concentrated in erythrocytes than in plasma. CPK was highly concentrated in the heart and the muscle, GOT was concentrated in the heart while the liver appeared to be a valuable source of GDH (as well as the kidney) and GPT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1975.tb04625.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Blood and liver enzymes in rainbow trout (Salmo gairdneri Rich.) with emphasis on their diagnostic use: Study of CCl4 toxicity and a case of Aeromonas infection (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 7 (1975), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of one intraperitoneal injection of 1.33 ml of CCl4 per kg of fish was studied. Two experimental series were performed and studied for 10 days (with diluent) and 24 h (pure CCl4) periods. LDH, GOT, GPT, GR, GDH, CPK, G-6-Pase, and AlkPase were studied. The activity of all enzymes in blood increased: LDH (four times the control), GOT (two times), GPT (three times); they reached a maximal activity 12 h after injection of diluted CCl4. The levels of some enzymes were also examined in the liver. With pure CCl4, maximal enzyme activity in blood occurred earlier (6 h). A 6 to 10 times increase was observed for GOT, GPT, LDH, GR, and GDH. Histopathological observations were correlated with these enzymes studies.An Aeromonas disease characterized by the destruction of the dermis, the exposure of the muscle, and by the presence of numerous petechiae in the liver enabled us to examine the relationships between naturally induced tissue damage and enzyme levels in blood. The levels of seven blood enzymes were determined and the most significant modifications were observed for LDH and CPK. which increased their concentration from 3 to 7 times respectively. A pyruvate saturation test demonstrated that LDH was probably from liver as it was observed after CCl4 poisoning. The contribution of such biochemical studies in fish research is evaluated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1975.tb04653.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Preliminary experiments on the artificial feeding of mugilidae (1970)
    Springer
    Helgoland marine research 20 (1970), S. 610-619 
    Details
    ISSN: 1438-3888
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary 1. A diet rich in proteins is necessary for the growth of very youngMugil auratus andM. capito (standard length: 21 mm). As soon as the natural change of diet occurs, a smaller quantity is sufficient. 2. The nature of proteins, incorporated in the food, does not seem to influence conversion rates. 3. Under conditions of complete starvation, a solution of urea at a concentration of 4 g/l reduces weight loss in youngMugil capito (1.5 % instead of 23 % loss after 9 day's starvation). 4. At 15° C, 10 gMugil auratus require a daily maintenance ration of 0.92 Cal; a ration of 1.8 Cal facilitates a higher growth efficiency (7 % in 3 weeks). 5. The addition of non-assimilable components reduces the rate of utilization of assimilable food substances. 6. Salinity influences food conversion rate. A salinity of 20 ‰ affords maximum growth and highest efficiency of food turnover.
    Notes: Extrait Les principales caractéristiques de la nutrition artificielle sont étudiées chez de jeunesMugil auratus etMugil capito. Nous soulignons l'importance de la teneur en protéines dans l'aliment chez les alevins et la nécessité des glucides chez les individus plus âgés. L'urée est susceptible d'être utilisée comme source d'azote non indispensable pour maintenir un poids constant chez des animaux à jeûn. La ration journalière d'entretien à 15° C s'élève à 0,92 Cal. pour un poisson de 10 g et une ration de 1,88 Cal. assure une croissance en poids de 7,3 % en trois semaines. L'incorporation de cellulose dans l'aliment diminue la croissance en élevant le taux de conversion. Au cours d'expériences de nutrition à salinités contrôlées, il est apparu que l'élevage en eau de mer diluée (S=20 ‰) permet une meilleure utilisation de l'aliment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01609933
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    Paper (German National Licenses)
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