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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 23 (1977), S. 259-269 
    ISSN: 1432-0827
    Keywords: Amorphous calcium phosphate ; Apatite ; Calcification ; Octacalcium phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The maturation of calcium phosphate crystals formed by the conversion of spontaneously precipitated amorphous calcium phosphate (ACP) was studied in aqueous media at temperatures ranging from 20° to 37°. Reaction pH was kept at 7.4 with either Hepes buffer or by the pH-stat addition of base. Reaction kinetics were followed by monitoring solution calcium and total phosphate, and, in the pH-stat controlled reaction, by recording the amount of KOH needed to maintain pH. Reaction products were examined chemically and by X-ray diffraction and transmission electron microcopy. The first crystals to form deviated markedly from apatite in morphology, composition, structure, and solubility. They were extremely thin and flaky in appearance, had a low Ca/P molar ratio (1.4), contained an appreciable amount of acid phosphate (16%), and had an exceptionally largea-axis (10.5 Å vs. 9.4 Å for apatite). With maturation, the crystals became thicker but smaller in lateral dimensions, more apatitelike in structure and composition, and less soluble. However, this ripening of the crystals was accompanied by unusual inflections in the solution Ca and total PO4 curves, and, in the case of the pH-stat experiments, in the OH consumption profiles as well. These anomalous post-ACP solution changes suggest that a phase change had taken place during crystal maturation. Although the observed structural and compositional changes are not inconsistent with the perfection of an initially defective apatite, the changes in crystal morphology and the anomalous behavior of the reaction solution may more accurately reflect a conversion of the ACP first into an OCP-like crystalline phase which subsequently hydrolyzes into apatite.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 3 (1969), S. 266-273 
    ISSN: 1432-0827
    Keywords: Calcification ; Arteries ; Membranes ; Elastic tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Avec la modification d'une méthode de von Kossa nous avons macroscopiquement representé les dessins de calcification de la membrane élastique intérieure, ainsi que les grosses artères du bassin, des artères des extrémités inferieures, des artères du bras, de la rate et des reins. Dans les artères du type élastique, dans les A.a. ilicae communes et internae, se présentaient les dégénérations calcaires de la membrane élastique intérieure sous forme d'incrustations noires arrondies ou polygonales qui ont été formes en groupes. Elles étaient toujours présentes chez des enfants agés de plus de trois mois et chez des aux adultes. Dans les artères musculaires, se montraient les parties calcifiées de la membrane élastique intérieure en forme de «bandes de calcaire» noires et groupes en paires le long des bordures des fissures de la membrane. Dans le cas où existait une calcinose forcée, on pouvait montrer avec cette méthode tout le système de fissures de la mambrane élastique intérieure. Chez tous les morts âgés de 10 à 20 ans, nous avons trouvé des bandes de calcaire dans les artères musculaires des extrémités inferieures. Ces bandes de calcaire ont été constatées aussi dans des personnes plus âgées. Au point de la fusion des bandes de calcaire et des incrustations calcaire polygonales des dépôts calcaires en forme de feuille («feuilles de calcaire») se forment dans la membrane elastique. Les bandes de calcaire et les feuilles de calcaire représentent des points de cristallisation pour des dépôts calcaire granuleux. Ils se forment dans la plupart des cas sur le la côté externe tourné vers la media.
    Abstract: Zusammenfassung Mit einer modifizierten von Kossa-Methode wurden die Verkalkungsmuster der inneren elastischen Membran der großen Beckenarterien, der Arterien der unteren Extremität, der Oberarm-, Milz-und Nierenarterien makroskopisch dargestellt. In den Arterien vom elastischen Typ, i.e. in den Aa. ilicae communes et internae, erschienen die Verkalkungen der inneren elastischen Membran als schwarze rundliche oder polygonale Inkrustationen, die zu Gruppen angeordnet waren. Sie wurden häufig bereits bei Neugeborenen festgestellt. Bei Kindern, die älter waren als 3 Monate und bei Erwachsenen lagen sie stets vor. — In den muskulären Arterien traten die verkalkten Anteile der inneren elastischen Membran makroskopisch als schwarze paarweise angeordnete „Kalkbänder” entlang den Rändern vorgebildeter Membranspalten auf. Bei ausgeprägter Calcinose konnte mit der angewandten Methode das gesamte Spaltensystem der inneren elastischen Membran dargestellt werden. Kalkbänder wurden in den muskulären Arterien der unteren Extremität bei allen 10–20 Jahre alten Verstorbenen vorgefunden und waren auch in den nachfolgenden Altersstufen stets vorhanden. Beim Zusammenfluß von Kalkbändern und polygonalen Kalkinkrustationen entstehen in der inneren elastischen Membran folienartige Kalkablagerungen („Kalkfolien”). Die Kalkbänder und Kalkfolien stellen Kristallisationspunkte für körnige Kalkablagerungen dar, die zumeist an ihrer äußeren, der Media zugekehrten Seite entstehen.
    Notes: Abstract Calcification patterns of the internal elastic membrane of the main pelvic arteries, lower limb arteries, brachial, splenic and renal arteries were demonstrated grossly by a modified von Kossa technique. In the elastic segment in the common and internal iliac arteries, the membrane calcification appeared as groups of roundish or polygonal incrustations. They were found frequently in newborns, and were always present in infants of more than three months, as well as in adults. In the muscular arteries, the calcified parts of the internal elastic membrane appeared grossly as pairs of bands (“calcific bands”) along the edges of the pre-existing gaps in this membrane. When calcification was pronounced, the whole pattern of the membrane gaps could be demonstrated by the method used in this study. Calcific bands were found in the muscular arteries of the lower limbs in all 10–20 year-old subjects, and were always present in the older age groups. The confluence of calcific bands or polygonal membrane incrustations found in the iliac arteries lead to sheet-like membrane calcification. The calcific bands and sheets represent crystallizing points for grain-like calcific deposits, which appear later on the medial surface.
    Type of Medium: Electronic Resource
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