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  • 1975-1979  (1)
  • 1965-1969  (3)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 66 (1969), S. 389-395 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A strain of Aspergillus niger adapted to grow on gluconate is cultivated on a medium containing gluconic acid lactone as the sole carbon source. Nutritional requirements of the adapted mold were studied. The organism shows optimum growth at 30° C in a medium containing per litre 20 g of gluconic acid lactone, 800 mg of NH4NO3, 100 mg of MgSO4, 7 H2O and 270 mg of KH2PO4 having pH 2.0. Mycelial growth reaches its maximum on the third day. Comparative growth responses of the adapted and parent strains on gluconate, glucose and sucrose were studied.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 66 (1969), S. 406-412 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Phosphogluconate dehydrogenase was purified about 20 fold from an adapted strain of Aspergillus niger cultivated on gluconate as the sole carbon source. The enzyme showed pH optimum between 7.0 to 7.6. The enzyme was activated by glycyl glycine buffer and magnesium ions. Mn++, Ba++, Ca++ cysteine and reduced glutathione activated the enzyme, while Co++, Zn++, Cu++, Hg++, PCMB and monoiodoacetate inhibit the enzyme. K m values for the substrates, 6-phosphogluconate and NADP, were found to be 1.66×10−4 m and 8.3×10−5 m respectively.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 66 (1969), S. 396-405 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A citric acid accumulating strain of Aspergillus niger adapted to grow on gluconic acid lactone as sole carbon source was studied with regards to the enzymatic changes occurring during its adaptive growth and compared with those of the parent strain cultivated on sucrose. Glucose oxidase, glucose dehydrogenase, gluconate dehydrogenase and enzymes of Entner Doudoroff pathway could not be detected in the strain. Gluconokinase was detected in the strain and its inducible nature was established. An adaptive increase of gluconokinase, phosphogluconate dehydrogenase and ribose phosphate isomerase was observed. While the levels of the above enzymes were significantly higher, that of glucose 6-phosphate dehydrogenase were significantly low indicating the repression of the enzyme. Levels of hexokinase and fructose diphosphate aldolase remained more or less the same as that of parent strain. It was inferred from the results that gluconate metabolism occurs via the pentose phosphate pathway following initial phosphorylation.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 291-293 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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