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  • 1975-1979  (3)
  • 1960-1964  (3)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 86 (1979), S. 0 
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Normal term human decidua-chorion, amnion and placenta were examined for possible synthesis of prolactin (PRL) by organ culture of tissue fragments in medium M199. Before incubation, higher PRL concentrations were found in decidua-chorion (27.7±5.7 ng/mg protein; mean±SE) and amnion (19.7±6.9 ng/mg protein) than in placenta (3.1±0.5 ng/mg protein; p 〈0.005). Incubated deciduachorion released significantly more PRL into culture medium than did amnion or placenta. After 72 hours culture, the PRL concentration of decidua-chorion remained high (17.9±6.4 ng/mg protein); that in amnion (2.3±1.9 ng/mg protein) fell to approach that of placenta (1.4±1.0 ng/mg protein). In further studies, fragments of decidua-chorion were incubated in medium RPMI 1640 without or with puromycin (0.04 mM) or cycloheximide (0.1 mM). A decrease in medium PRL concentration was observed within four hours of adding these inhibitors of protein synthesis. At four hours, the PRL content of decidua-chorion cultured in puromycin (12.4±3 8 ng/mg protein) or cycloheximide (11.9±2.9 ng/mg protein) was lower than tissue incubated in medium alone (25.5±4.1 ng/mg protein; p 〈0.05). Comparable differences in tissue content and medium concentration were also seen at 24 and 72 hours. We conclude that human decidua-chorion synthesizes a protein immunologically simjlar to PRL and that this tissue may represent the principal source of amniotic fluid PRL.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 189 (1961), S. 213-214 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Its theory is not clear and has been discussed by Garner and Nissan3, Rivlin4, Reiner et al.* and Roberts5. The effect occurs only rarely and has been observed in highly viscous solutions of rubber in benzene8, certain soaps in hydrocarbon solvents3, over-aged sweetened condensed milk2 and certain ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 265 (1977), S. 642-643 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Placentae and amniotic membranes were collected from normal pregnancies of 12-40 weeks gestation, which had been terminated for psychiatric reasons in the second trimester by hysterotomy or had been delivered vaginally either prematurely or at term in the third trimester. The tissue was immediately ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 264 (1976), S. 648-650 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The neural tissues examined comprised cerebellum and cerebrum from rat, mouse, rabbit, guinea pig, sheep and chicken, and spinal cord from rat, mouse and chicken. Synaptosome8- and myelin9-enriched fractions were also isolated from chicken forebrain; effective enrichment was established by electron ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 188 (1960), S. 238-239 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] White mice of both sexes aged 3 4 weeks were used in five successive experiments each comprising 60 animals. This age-group was chosen because Ponseti and Shepard3 found that the most marked aortic lesions were produced in the rat when treatment with a lathyrous diet was begun before the age of 4 ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 55 (1964), S. 228-228 
    ISSN: 1437-1596
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Type of Medium: Electronic Resource
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