ISSN:
1573-4927
Keywords:
isozymes
;
α-d-mannosidase
;
somatic cell hybrids
;
phosphohexose isomerase, chromosome 19, lysosomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Two types of α-d-mannosidase isozymes are present in human white blood cells, human diploid fibroblasts, and HeLa cells. One of these (the S isozyme) constitutes the major α-d-mannosidase of the human cells, has a pH optimum of 4.4, and is associated with lysosomes. The other (the F isozyme) is most active at pH 6, is acid labile, and is located in the soluble portion of the cytoplasm. The expression of human lysosomal α-d-mannosidase was examined in man-hamster hybrid clones, and was found to be concordant with that of phosphohexose isomerase in 54 of 55 primary clones. A locus specifying human lysosomal α-d-mannosidase has therefore been assigned to chromosome 19.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00520191
Permalink
