ISSN:
1432-1912
Keywords:
Substantia nigra
;
5-Hydroxytryptamine
;
α-Flupenthixol
;
Substance P
;
Dopamine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The effects of GABA, substance P and dopamine on the release of newly synthesized 3H-5-HT were investigated, using slices of rat substantia nigra superfused with l-3H-tryptophan in vitro. GABA (50 μM) had no inhibitory effect on the potassium-evoked-release of 3H-5-HT. Substance P (50 μM) and eledoisin (50 μM) stimulated the spontaneous release of 3H-5-HT. This effect seems to be indirect and is possibly mediated by dopaminergic neurones, since the dopamine antagonist drug α-flupenthixol (1 μM) abolished the substance P-evoked release of 5-HT. Furthermore, it was found that substance P (10 μM) stimulated 3H-dopamine release from nigral slices in vitro and the dopaminergic agonist apomorphine (50 μM) also stimulated 3H-5-HT release. Substance P may, therefore, activate nigral dopaminergic neurones which then release dopamine from their dendrites. The release of dopamine may in turn stimulate 5-HT release from terminals of the raphe-nigral pathway.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00507968
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