ISSN:
1432-072X
Keywords:
Trichoderma viride
;
Fungal Protoplasts
;
Protoplasts Formation
;
Protoplasts Regeneration
;
Abortive Tubes
;
Lytic System
;
Streptomyces venezuelae RA
;
Micromonospora chalcea
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract High yields of protoplasts from the 18-hr old mycelium of Trichoderma viride were obtained by using the lytic system, produced by Streptomyces venezuelae RA and Micromonospora chalcea grown on a synthetic medium containing laminarin and chitin, when 0.7 M MgSO4 or (NH4)2SO4 were used as osmotic stabilizers. Regeneration of these protoplasts occurred through the production of an abortive tube and direct germination of the protoplasts. Regeneration could also take place in the medium used to produce protoplasts, but the process was different in many details.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00436350
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