ISSN:
1432-2048
Keywords:
Cell wall
;
Chlamydomonas
;
Glyco-protein
;
Hydroxyproline
;
Self assembly
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have investigated the in vitro reassembly of the salt soluble, hydroxyproline rich, glycoproteins from the cell wall of Chlamydomonas reinhardii, into structured cell wall fragments. We have devised an assay which has been used to follow the reassembly of the unfractionated and fractionated (2BI and 2BII) cell wall glycoproteins. Reassembly has a pH optimum of 5, a temperature optimum of 20°C, and the final size of the reassembled fragments appears to be promoted by the minor component 2BI. Periodate oxidation experiments show that sugar residues, in particular mannose, are important for accurate reassembly. Using electron microscopy, the structure of the reassembled products has been elucidated, as have intermediate stages in the reassembly process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392922
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