ISSN:
1432-2013
Keywords:
Calcium uptake
;
Insulin release
;
Islets of Langerhans
;
Lanthanum
;
Magnesium
;
Pancreatic islets
;
Potassium
;
Sodium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Microdissected pancreatic islets of noninbredob/ob-mice were used to study ionic effects on the lanthanum-nondisplaceable45Ca2+ uptake by islet cells. Omission of Mg2+ from the incubation medium had no effect, but the45Ca2+ uptake was increased by omission of Na+ and decreased by omission of K+. Excess Mg2+ (1.2–15 mM) inhibited and excess K+ (4.7–25 mM) stimulated the45Ca2+ uptake in a concentration-dependent manner. Stimulation of45Ca2+ uptake in Na+-deficient islets was associated with an enhancement of the basal insulin release. Total abolishment of glucose-stimulated45Ca2+ uptake in K+-deficient islets did not preclude a significant secretory response to glucose. It is concluded that the lanthanum-nondisplaceable45Ca2+ uptake shows a partial correlation to insulin release.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00584440
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