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  • 1975-1979  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Investigation of the unit operations involved in the continuous flow isolation of β-galactosidase from Escherichia coli (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 20 (1978), S. 159-182 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A 1000 liter fermentor has been used to produce a continuous feed of Escherichia coli containing a high level of β-galactosidase. We have investigated the individual unit operations for the isolation of the enzyme: cell disruption, nucleic acid removal, protein precipitation, and solid-liquid separation after each stage. Using the information obtained we have been able to operate a semicontinuous process which when fully continuous would yield 100 g protein/hr, comprising 23% β-galactosidase.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260200202
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Enzyme immobilization on fibrin (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 133-139 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260180112
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Application of Bender's salts to the synthesis of S-vinyl-O-tert-alkylthiocarbonates (1975)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 13 (1975), S. 105-118 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Three thermally labile Bender's salts were synthesized and utilized to effect quantitative SN2 displacement of primary halides to form tert-alkylthiocarbonate derivatives. Selective displacement of bromide from 1,2-bromochloroethane by either VI or VII followed by dehydrohalogenation with potassium tert-butoxide yielded S-vinyl-O-tert-butylthiocarbonate (I) and S-vinyl-O-tert-amylthiocarbonate (X), two excellent vinyl monomer precursors for polymercaptan, in 62 and 51% yield, respectively. Reaction of VI with vinylbenzyl chloride yielded S-(vinylbenzyl)-O-tert-butylthiocarbonate which polymerized in the presence of free radical initiators to produce a poly(vinylbenzyl mercaptan) precursor. Dilatometric studies of the homopolymerization of I demonstrated that the polymerization rate was proportional to [I]0.765 and [M]1.7, respectively; the monomer exhibited a high chain transfer constant (CM = 3.9 × 10-3).
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1975.170130111
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    Paper (German National Licenses)
    Fulltext
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